The detection of 20( S)-hydroxycholesterol in extracts of rat brains and human placenta by a gas chromatograph/mass spectrometry technique

The presence of 20( S)-hydroxycholesterol in rat brains and human placenta has been established using the gas chromatography/mass spectrometry (GC/MS) select ion monitoring (SIM) technique. Identification was ensured by three criteria: the specific retention time when the compound emerges from the g...

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Bibliographic Details
Published inThe Journal of steroid biochemistry and molecular biology Vol. 85; no. 1; pp. 57 - 61
Main Authors Lin, Ying Yong, Welch, Margaret, Lieberman, Seymour
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.05.2003
Elsevier Science
Subjects
20( S)-Hydroxycholesterol
Analytical biochemistry: general aspects, technics, instrumentation
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Brains
Fundamental and applied biological sciences. Psychology
Oxysterols
Placenta
Steroidogenesis
Brains
20( S)-Hydroxycholesterol
Steroidogenesis
Placenta
Oxysterols
Human
Rat
Fetal membrane
Rodentia
Central nervous system
In vitro
Gas chromatography
Vertebrata
Mammalia
Detection
Mass spectrometry
Brain (vertebrata)
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Summary:The presence of 20( S)-hydroxycholesterol in rat brains and human placenta has been established using the gas chromatography/mass spectrometry (GC/MS) select ion monitoring (SIM) technique. Identification was ensured by three criteria: the specific retention time when the compound emerges from the gas chromatogram and the two m/ z ions (201 and 461 amu) which are characteristic of its mass spectrum. The possible role of 20( S)-hydroxycholesterol in steroid hormone biosynthesis and in other biological processes is discussed.
ISSN:0960-0760
1879-1220
DOI:10.1016/S0960-0760(03)00137-7