Cloning and Expression of Synthetic Plantaricin F Gene

Antimicrobial activity of certain species of Lactobacillus bacteria were known to produce a certain type of bacteriocin called Plantaricin (Pln). In this study, the PlnF (plnF) gene (666 bp) encoding plantaricin was synthetically constructed based on a sequence of predefined and valuable amino acid...

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Bibliographic Details
Published inJournal of pure & applied microbiology : an international research journal of microbiology Vol. 17; no. 1; pp. 289 - 297
Main Authors Madhi, Khadeeja S., Khudor, Mohammed H., Othman, Rasha M.
Format Journal Article
LanguageEnglish
Published Oriental Scientific Publishing Company 01.03.2023
Journal of Pure and Applied Microbiology
Subjects
Analysis
bacteriocin
Cloning
Escherichia coli
Genes
lactobacillus
Pets
plantaricin f. expression
synthetic gene
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Summary:Antimicrobial activity of certain species of Lactobacillus bacteria were known to produce a certain type of bacteriocin called Plantaricin (Pln). In this study, the PlnF (plnF) gene (666 bp) encoding plantaricin was synthetically constructed based on a sequence of predefined and valuable amino acid compositions and subcloned into pET-28 (+) expression vector. Thereafter transformation of recombinant vector into BL21 (DE3) competent E. coli was achieved and to verify the result of cloning, the PCR colony technique was conducted. The expression of the inserted gene was analyzed by the SDS-polyacrylamide gel electrophoresis method, which detected a band of approximately 30 KD molecular weight for interested protein as a natural bioactive bacteriocin.
ISSN:0973-7510
2581-690X
DOI:10.22207/JPAM.17.1.18