An alkali stable cellulase by chemical modification using maleic anhydride

An alkali stable cellulase was obtained by chemical modification with 0.5 M maleic anhydride at 2–4°C and pH 8.0 for 25 min. The cellulase so modified showed a marginally better activity in alkaline range as compared to native cellulase. It had a half-life of 462.0, 433.13, 128.33 and 144.38 h vis-à...

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Bibliographic Details
Published inCarbohydrate polymers Vol. 47; no. 2; pp. 137 - 141
Main Authors Bund, Rajesh K, Singhal, Rekha S
Format Journal Article
LanguageEnglish
Published Oxford Elsevier Ltd 01.02.2002
Elsevier Science
Subjects
Akali stable cellulase
Biological and medical sciences
Biotechnology
Chemical modification
Chemical synthesis for preparing modified enzymes, enzyme fragments and enzyme analogs
Enzyme engineering
Fundamental and applied biological sciences. Psychology
Maleic anhydride
Methods. Procedures. Technologies
Production of selected enzymes
Akali stable cellulase
Chemical modification
Maleic anhydride
Stability
Enzymatic activity
Enzyme
Cellulase
Glycosidases
Industrial application
pH
Hydrolases
Basic medium
O-Glycosidases
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Summary:An alkali stable cellulase was obtained by chemical modification with 0.5 M maleic anhydride at 2–4°C and pH 8.0 for 25 min. The cellulase so modified showed a marginally better activity in alkaline range as compared to native cellulase. It had a half-life of 462.0, 433.13, 128.33 and 144.38 h vis-à-vis 13.08, 121.58, 126.0 and 187.30 for native unmodified enzyme at a pH of 8.0, 9.0, 10.0 and 11.0, respectively, at 50°C. These results could have promising applications in textiles and detergents.
ISSN:0144-8617
1879-1344
DOI:10.1016/S0144-8617(01)00182-5