A novel colorimetric and far-red emission ratiometric fluorescent probe for the highly selective and ultrafast detection of hypochlorite in water and its application in bioimaging

• Published in: Analyst (London) Vol. 145; no. 3; pp. 939 - 945
• Main Authors: Zhang, Yuanyuan, Ma, Yufan, Wang, Zhuo, Zhang, Xueyan, Chen, Xin, Hou, Shicong, Wang, Hongmei
• Format: Journal Article
• Language: English
• Published: England Royal Society of Chemistry 03.02.2020
• Subjects: Aqueous solutions; Arthritis; Atherosclerosis; Biomonitoring; Colorimetry; Fluorescent indicators; Medical imaging; Selectivity
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/c9an02034a

Hypochlorous acid (HOCl)/hypochlorite (OCl − ), an important reactive oxygen species, plays a number of important roles in various physiological processes. However, abnormal levels of OCl − can cause many serious diseases, such as atherosclerosis, rheumatoid arthritis, cardiovascular disease, and cancer. The development of efficient methods to monitor OCl − in biological systems is therefore of particular importance. Thus, we herein report a novel isophorone-based fluorescent probe, i.e. , DCOH-FR-OCl , for OCl − detection. This probe was found to exhibit colorimetric and far-red ratiometric fluorescence response signals, excellent selectivity and sensitivity for OCl − (detection limit, 88.06 nM), a remarkably large Stokes shift (158 nm), an ultrafast response (completed within 3 s), perfect photostability, and good water solubility (100% in aqueous media). DCOH-FR-OCl , as we know, is the first probe that can detect OCl − by using two different response signals at an ultrafast speed with a large Stokes shift in fully aqueous media. Furthermore, DCOH-FR-OCl can be successfully employed in the real-time imaging of endogenous and exogenous OCl − in living cells. Hypochlorous acid (HOCl)/hypochlorite (OCl − ), an important reactive oxygen species, plays a number of important roles in various physiological processes.