Efficient synthesis of deuterium-labelled Danshensu for quantitative bioanalysis

• Published in: Journal of radioanalytical and nuclear chemistry Vol. 325; no. 1; pp. 167 - 173
• Main Authors: Li, Yi, Xu, Honglei, He, Kun, Xiong, Liyan, Zhang, Chengzhong, Wang, Tingfang, Zhang, Chuan, Chen, Min
• Format: Journal Article
• Language: English
• Published: Cham Springer International Publishing 01.07.2020; Springer; Springer Nature B.V
• Subjects: Angina pectoris; Chemistry; Chemistry and Materials Science; Deuterium; Diagnostic Radiology; Dilution; Hadrons; Heavy Ions; In vivo methods and tests; Inorganic Chemistry; Medical research; Medicine, Botanic; Medicine, Experimental; Medicine, Herbal; Nuclear Chemistry; Nuclear Physics; Physical Chemistry; Danshensu; Deuterium-labelled; Stable isotope-labelled internal standards; Quantitative bioanalysis
• ISSN: 0236-5731; 1588-2780
• DOI: 10.1007/s10967-020-07190-w

Danshensu (DSS) is a bioactive compound isolated from the Chinese herb Radix Salviae Miltiorrhizae , which has been applied for the treatment of angina pectoris. The stable isotope-labelled DSS is required to be used for clinical studies and biological sample analysis. This study aims to synthesize deuterium-labelled DSS to change the status of DSS without internal standard, and to accelerate the research progress of DSS in vivo. An effective and convenient method for the synthesis of deuterium-labelled DSS was developed using Pd/C as a catalyst and D 2 O as a deuterium source. Our results showed that deuterium-labelled DSS with high deuterium incorporation has been achieved and could be used as internal standards in stable isotopic dilution MS/MS studies to accurately quantify DSS levels in biologic samples.