The 3 ′ termini of small RNAs in Trypanosoma brucei
Post-transcriptional 3 ′ end formation is an essential step in the maturation of most small RNAs. Knowledge of the precise 3 ′ ends of mature RNAs is essential for defining 3 ′-processing activities. We have mapped the mature 3 ′ ends of Spliced Leader RNA, the U1, U2, U4, U5, and U6 small nuclear R...
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Published in | FEMS microbiology letters Vol. 236; no. 1; pp. 73 - 78 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Elsevier B.V
01.07.2004
Blackwell |
Subjects | |
Online Access | Get full text |
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Summary: | Post-transcriptional 3
′ end formation is an essential step in the maturation of most small RNAs. Knowledge of the precise 3
′ ends of mature RNAs is essential for defining 3
′-processing activities. We have mapped the mature 3
′ ends of Spliced Leader RNA, the U1, U2, U4, U5, and U6 small nuclear RNAs, the U3 small nucleolar RNA, and the 5S and 5.8S ribosomal RNAs by ligation-mediated PCR in
Trypanosoma brucei. With the exception of U5, two classes of 3
′ ends were observed: flush with the base of a stem–loop structure, and 1–2 nt extended from a stem–loop. Multiple mechanisms and structural features are likely to influence 3
′ end maturation of RNAs. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0378-1097 1574-6968 |
DOI: | 10.1016/j.femsle.2004.05.020 |