Vitamin E promotes ovine Sertoli cell proliferation by regulation of genes associated with cell division and the cell cycle

• Published in: Animal biotechnology Vol. 33; no. 2; pp. 392 - 400
• Main Authors: Gao, Yuefeng, Lu, Wei, Jian, Luyang, Machaty, Zoltan, Luo, Hailing
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis Ltd 01.04.2022
• Subjects: Animals; Cell cycle; Cell Cycle - genetics; Cell Division; Cell growth; Cell proliferation; Cell Proliferation - genetics; Cyclin B1; G1 phase; Gene expression; Gene regulation; Genes; Male; Myc protein; Peroxisome proliferator-activated receptors; Reproduction; Sertoli Cells; Sheep; Sheep - genetics; Tocopherol; Transcription; Vitamin E; Sertoli cell; proliferation; Vitamin E; cell cycle; sheep
• ISSN: 1049-5398; 1532-2378
• DOI: 10.1080/10495398.2020.1788044

The effect of Vitamin E on the proliferation of ovine Sertoli cells was investigated. Sertoli cells were isolated and treated with various amounts of Vitamin E (0 μM, 400 μM, 800 μM, 1000 μM, 1200 μM, 1400 μM and 1600 μM) for 24 h. We found that at the concentration of 1200 μM, Vitamin E promoted Sertoli cell proliferation very effectively. It also increased the proportion of cells in the G1 phase while reduced that in the S and G2/M phases, suggesting that its effect on Sertoli cell proliferation is achieved by enhancing progression through the cell cycle. In addition, Vitamin E significantly up-regulated the transcript level of the PDPN, BMP6, AMPKα, GSK3β, Myc, and CDK6 genes and down-regulated that of PPARγ, Cyclin B1 and CDK4 as determined by qRT-PCR. Western blot analysis revealed that the expression of BMP6 and PDPN was also upregulated at the protein level, in accordance with the results of the qRT-PCR. Taken together, Vitamin E promoted Sertoli cell proliferation by affecting the expression of genes that regulate cell division and the cell cycle; this indicates that it can have a positive effect on sheep reproductive performance.