Production of pulchelin E in hairy roots, callus and suspension cultures of Rudbeckia hirta L

• Published in: Plant science (Limerick) Vol. 163; no. 1; pp. 91 - 100
• Main Authors: Łuczkiewicz, Maria, Zárate, Rafael, Dembińska-Migas, Wanda, Migas, Piotr, Verpoorte, Robert
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 01.07.2002; Elsevier Science
• Subjects: Biological and medical sciences; Biotechnology; Densitometry; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Hairy roots; In vitro cultures; In vitro propagation: entire plant regeneration from tissues and cell cultures; Methods. Procedures. Technologies; Plant cells and fungal cells; Plant physiology and development; Product release; Rudbeckia hirta; Sesquiterpene lactones; TIBA; Tissue cultures, protoplasts; TIBA, 2,3,5-triiodobenzoic acid; Product release; G i, growth index; IBA, indole-3-butyric acid; Rudbeckia hirta; Hairy roots; In vitro cultures; SH, Schenk & Hildebrandt; FW, fresh weight; Sesquiterpene lactones; TIBA; DW, dry weight; NAA, α-naphthaleneacetic acid; 2,4-D, 2,4-dichlorophenoxyacetic acid; IAA, indole-3-acetic acid; Densitometry; Hairy root; Cell culture; Callus; Genetic transformation; Lactone; Arenoic acid; Biosynthesis; Growth regulator treatment; Compositae; Dicotyledones; Sesquiterpenes; Angiospermae; Production; Bacteria; Flower crop; Plant growth substance; Genetic engineering; Spermatophyta; Rhizobiaceae; Agrobacterium rhizogenes; Secondary metabolism
• ISSN: 0168-9452; 1873-2259
• DOI: 10.1016/S0168-9452(02)00065-1

Agrobacterium rhizogenes transformed hairy roots, callus and suspension cultures of Rudbeckia hirta produced pulchelin E, a compound of the sesquiterpene lactone group, which is not present in the intact plant. During the research the level of the investigated lactone was optimised yielding 8.3 mg g −1 in the callus, 9.1 mg g −1 in the suspension cultures and 14.7 mg g −1 in the transformed roots (per dry weight basis). The content of pulchelin E in the cultures was calculated by thin-layer chromatography method with densitometric detection. Pulchelin E production in R. hirta tissues occurred only under constant light and was correlated with the growth cycle. In suspension cultures pulchelin E was partly released into the medium; this was not observed in transformed root cultures. The exceptional influence of 2,3,5-triiodobenzoic acid (TIBA) on pulchelin E production was stated.