Production of pulchelin E in hairy roots, callus and suspension cultures of Rudbeckia hirta L
Agrobacterium rhizogenes transformed hairy roots, callus and suspension cultures of Rudbeckia hirta produced pulchelin E, a compound of the sesquiterpene lactone group, which is not present in the intact plant. During the research the level of the investigated lactone was optimised yielding 8.3 mg g...
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Published in | Plant science (Limerick) Vol. 163; no. 1; pp. 91 - 100 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Shannon
Elsevier Ireland Ltd
01.07.2002
Elsevier Science |
Subjects | |
Online Access | Get full text |
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Summary: | Agrobacterium rhizogenes transformed hairy roots, callus and suspension cultures of
Rudbeckia hirta produced pulchelin E, a compound of the sesquiterpene lactone group, which is not present in the intact plant. During the research the level of the investigated lactone was optimised yielding 8.3 mg g
−1 in the callus, 9.1 mg g
−1 in the suspension cultures and 14.7 mg g
−1 in the transformed roots (per dry weight basis). The content of pulchelin E in the cultures was calculated by thin-layer chromatography method with densitometric detection. Pulchelin E production in
R. hirta tissues occurred only under constant light and was correlated with the growth cycle. In suspension cultures pulchelin E was partly released into the medium; this was not observed in transformed root cultures. The exceptional influence of 2,3,5-triiodobenzoic acid (TIBA) on pulchelin E production was stated. |
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ISSN: | 0168-9452 1873-2259 |
DOI: | 10.1016/S0168-9452(02)00065-1 |