In vitro and in vivo germination of papaya ( Carica papaya L.) seeds

• Published in: Scientia horticulturae Vol. 91; no. 1; pp. 39 - 49
• Main Authors: Bhattacharya, J, Khuspe, S.S
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 30.11.2001; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Carica papaya L; Economic plant physiology; Embryo development. Germination; Fundamental and applied biological sciences. Psychology; Germination; Germination and dormancy; Growth and development; In vitro germination; In vivo germination; Papaya; Plant physiology and development; Seed; 2,4,5-T, 2,4,5-Trichloro phenoxyacetic acid; Papaya; Germination; MS, Murashige and Skoog medium; 2,4-D, 2,4-Dichloro phenoxyacetic acid; In vitro germination; Carica papaya L; NAA, Naphthalene acetic acid; GA 3, Gibberellic acid; TDZ, Thidiazuron; BAP, 6-Benzyl amino purine; Seed; In vivo germination; Adenine(N; Purine derivatives; Embryo; Auxin; 1-Naphthaleneacetic acid; Seed priming; Temperature effect; Caricaceae; Environmental factor; Dicotyledones; Angiospermae; Alkanoic acid; Plant growth substance; Chemical composition; Fruit tree; Pretreatment; Intraspecific comparison; benzyl; Carica papaya; Culture medium; 2,4-D; Soaking; Cytokinin; In vitro; In vivo; 2,4,5-T; Spermatophyta; Phytohormone; Aryloxyacetic acids; Cultivar
• ISSN: 0304-4238; 1879-1018
• DOI: 10.1016/S0304-4238(01)00237-0

The effect of various pre-sowing treatments on seed germination in soil of 10 widely cultivated cultivars of papaya were studied. Intact seeds, naked embryos, halved seeds and halved embryos were germinated under in vitro culture conditions and the effects of thidiazuron (TDZ), 6-benzyl amino purine (BAP), naphthalene acetic acid (NAA), 2,4-dichloro phenoxyacetic acid (2,4-D) and 2,4,5-trichloro phenoxyacetic acid (2,4,5-T) at different concentrations, temperature and light were examined. Without pretreatment, the percentage germination in soil varied between 3 and 71% (average 40.2%). Soaking for 24 h in 200 ppm GA 3 increased germination to 12–79% (average 56.5%). The difference between in vivo and in vitro seed germination was observed to be the lowest in honeydew (6.3%) and highest in Disco (68%). In general, in vitro culture conditions increased the percentage and rate of seed germination in all cultivars. An average maximum germination of 95.5% was obtained after 7–8 days for naked embryos when cultured in the light at 30°C on Murashige and Skoog’s medium (MS) supplemented with TDZ (1.0 μM/l).