Integrative multi-omics analysis reveals genetic and heterotic contributions to male fertility and yield in potato

• Published in: Nature communications Vol. 15; no. 1; pp. 8652 - 12
• Main Authors: Li, Dawei, Geng, Zedong, Xia, Shixuan, Feng, Hui, Jiang, Xiuhan, Du, Hui, Wang, Pei, Lian, Qun, Zhu, Yanhui, Jia, Yuxin, Zhou, Yao, Wu, Yaoyao, Huang, Chenglong, Zhu, Guangtao, Shang, Yi, Li, Huihui, Städler, Thomas, Yang, Wanneng, Huang, Sanwen, Zhang, Chunzhi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 05.10.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 38; 38/39; 45; 45/23; 631/208/720; 631/208/8; 631/449/2491; 631/449/711; Agricultural production; Chromosome Mapping; Crop yield; Diploids; Diploidy; Dominance; Fertility; Fertility - genetics; Gene expression; Gene Expression Regulation, Plant; Gene mapping; Genetic analysis; Genetic resources; Genetics; Genomics; Heredity; Heterosis; Humanities and Social Sciences; Hybrid Vigor - genetics; Inbreeding; Males; Metabolites; multidisciplinary; Multiomics; Phenotype; Plant Breeding; Population genetics; Potatoes; Quantitative Trait Loci; Science; Science (multidisciplinary); Solanum tuberosum - genetics; Vegetables
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-53044-4

The genetic analysis of potato is hampered by the complexity of tetrasomic inheritance. An ongoing effort aims to transform the clonally propagated tetraploid potato into a seed-propagated diploid crop, which would make genetic analyses much easier owing to disomic inheritance. Here, we construct and report the large-scale genetic and heterotic characteristics of a diploid F 2 potato population derived from the cross of two highly homozygous inbred lines. We investigate 20,382 traits generated from multi-omics dataset and identify 25,770 quantitative trait loci (QTLs). Coupled with gene expression data, we construct a systems-genetics network for gene discovery in potatoes. Importantly, we explore the genetic basis of heterosis in this population, especially for yield and male fertility heterosis. We find that positive heterotic effects of yield-related QTLs and negative heterotic effects of metabolite QTLs (mQTLs) contribute to yield heterosis. Additionally, we identify a PME gene with a dominance heterotic effect that plays an important role in male fertility heterosis. This study provides genetic resources for the potato community and will facilitate the application of heterosis in diploid potato breeding. An ongoing effort aims to transform the clonally propagated tetraploid potato to a seed-propagated diploid crop, but our understanding of it disomic inheritance is limited. Here, the authors report genetic basis of heterosis in the elite hybrid potato and identify a male fertility-related PME gene with dominance heterotic effect.