Assessment of salivary microbiota profile as a potential diagnostic tool for pediatric celiac disease

• Published in: Scientific reports Vol. 14; no. 1; pp. 16712 - 9
• Main Authors: Noruzpour, Asal, Gholam-Mostafaei, Fahimeh Sadat, Looha, Mehdi Azizmohammad, Dabiri, Hossein, Ahmadipour, Shokoufeh, Rouhani, Pejman, Ciacci, Carolina, Rostami-Nejad, Mohammad
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 19.07.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/326; 692/4020/1503; Adolescent; Autoimmune diseases; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Case-Control Studies; Celiac disease; Celiac Disease - diagnosis; Celiac Disease - microbiology; Child; Child, Preschool; Children; Cross-Sectional Studies; Diet, Gluten-Free; DNA sequencing; Dysbacteriosis; Dysbiosis - diagnosis; Dysbiosis - microbiology; Female; Gluten; Humanities and Social Sciences; Humans; Male; Microbiomes; Microbiota; Microorganisms; multidisciplinary; Oral dysbiosis; Patients; Pediatric patients; Pediatrics; RNA, Ribosomal, 16S - genetics; rRNA 16S; Saliva; Saliva - microbiology; Salivary microbiota; Science; Science (multidisciplinary); Celiac disease; Oral dysbiosis; Salivary microbiota; Pediatric patients
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-67677-4

The association between oral dysbiosis and celiac disease (CD) remains poorly understood, as does the impact of CD-associated dysbiosis on disease development or exacerbation. This study aims to investigate alterations in salivary microbial composition among children with CD. In this cross-sectional study, saliva samples from 12 children with active CD (A-CD group), 14 children with CD on a gluten-free diet (GFD), and 10 healthy control (HC) children were analyzed using DNA sequencing targeting the 16S ribosomal RNA. Both patients in A-CD and GFD groups showed a significant increase (p = 0.0001) in the Bacteroidetes phylum, while the Actinobacteria phylum showed a significant decrease (p = 0.0001). Notably, the Rothia genus and R.aeria also demonstrated a significant decrease (p = 0.0001) within the both CD groups as compare to HC. Additionally, the control group displayed a significant increase (p = 0.006) in R.mucilaginosa species compared to both CD patient groups. Distinct bacterial strains were abundant in the saliva of patients with active CD, indicating a unique composition of the salivary microbiome in individuals with CD. These findings suggest that our approach to assessing salivary microbiota changes may contribute to developing noninvasive methods for diagnosing and treating CD.