Stability and Mismatch Discrimination of DNA duplexes containing 2,6-Diaminopurine and 2-Thiothymidine Locked Nucleic Acid bases

Abstract Hybridization thermodynamics measured by differential scanning calorimetry (DSC) and UV spectroscopy (UVM) are reported for 8- and 14-mer oligonucleotides containing two new LNA bases: 2,6 diaminopurine (D) and 2-thiothymidine (2sT). Oligonucleotides containing D or 2sT bases are shown to h...

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Bibliographic Details
Published inNucleic Acids Symposium Series Vol. 52; no. 1; pp. 245 - 246
Main Authors Hughesman, Curtis B., Turner, Robin F.B., Haynes, Charles
Format Journal Article
LanguageEnglish
Published England Oxford University Press 01.09.2008
Subjects
2-Aminopurine - analogs & derivatives
2-Aminopurine - chemistry
Base Pair Mismatch
Calorimetry, Differential Scanning
DNA - chemistry
Oligonucleotide Probes - chemistry
Oligonucleotides - chemistry
Spectrophotometry, Ultraviolet
Thermodynamics
Thymidine - analogs & derivatives
Thymidine - chemistry
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Summary:Abstract Hybridization thermodynamics measured by differential scanning calorimetry (DSC) and UV spectroscopy (UVM) are reported for 8- and 14-mer oligonucleotides containing two new LNA bases: 2,6 diaminopurine (D) and 2-thiothymidine (2sT). Oligonucleotides containing D or 2sT bases are shown to have enhanced stability and improved discrimination for several of the possible mismatched base pairs.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0261-3166
1746-8272
DOI:10.1093/nass/nrn124