Phalloidin and DNase I-bound F-actin pointed end structures reveal principles of filament stabilization and disassembly

• Published in: Nature communications Vol. 15; no. 1; pp. 7969 - 12
• Main Authors: Boiero Sanders, Micaela, Oosterheert, Wout, Hofnagel, Oliver, Bieling, Peter, Raunser, Stefan
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 11.09.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 101/28; 631/45/535; 631/535/1258/1259; 631/80/128/1276; Actin; Actin Cytoskeleton - metabolism; Actins - metabolism; Animals; Binding; Conformation; Cryoelectron Microscopy; Deoxyribonuclease; Deoxyribonuclease I - chemistry; Deoxyribonuclease I - metabolism; Depolymerization; Dismantling; Filaments; Heat treating; Humanities and Social Sciences; Models, Molecular; multidisciplinary; Phalloidin; Phalloidine - chemistry; Phalloidine - metabolism; Protein Binding; Protein Conformation; Rabbits; Science; Science (multidisciplinary)
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-52251-3

Actin filament turnover involves subunits binding to and dissociating from the filament ends, with the pointed end being the primary site of filament disassembly. Several molecules modulate filament turnover, but the underlying mechanisms remain incompletely understood. Here, we present three cryo-EM structures of the F-actin pointed end in the presence and absence of phalloidin or DNase I. The two terminal subunits at the undecorated pointed end adopt a twisted conformation. Phalloidin can still bind and bridge these subunits, inducing a conformational shift to a flattened, F-actin-like state. This explains how phalloidin prevents depolymerization at the pointed end. Interestingly, two DNase I molecules simultaneously bind to the phalloidin-stabilized pointed end. In the absence of phalloidin, DNase I binding would disrupt the terminal actin subunit packing, resulting in filament disassembly. Our findings uncover molecular principles of pointed end regulation and provide structural insights into the kinetic asymmetry between the actin filament ends. The pointed end of actin filaments is a dominant site of actin depolymerization. Here, the authors show how the actin modulators phalloidin and DNase I interact with the pointed end to either stabilize its arrangement or to promote its disassembly.