Interaction between plant-specific transcription factors TCP and YABBY expressed in the tendrils of the melon Cucumis melo

• Published in: Scientific reports Vol. 14; no. 1; pp. 22818 - 12
• Main Authors: Mizuno, Shinji, Masuda, Chiho, Otsuka, Ayami, Kishimoto, Nana, Kameyama, Chisato, Kamiyoshihara, Yusuke, Mitsuzawa, Hiroshi
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.10.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/449; 631/449/1659; 631/449/2491; 631/449/2653; Cucumis melo; Cucumis melo - genetics; Cucumis melo - metabolism; Cytotoxicity; Frameshift mutation; Gene Expression Regulation, Plant; Gene mapping; Genomic analysis; Humanities and Social Sciences; Lymphocytes T; Melon; multidisciplinary; Mutation; Peptide mapping; Plant cells; Plant Proteins - genetics; Plant Proteins - metabolism; Protein Binding; Science; Science (multidisciplinary); TCP; Tendril development; Transcription factor; Transcription factors; Transcription Factors - genetics; Transcription Factors - metabolism; Two-Hybrid System Techniques; YABBY; Yeast two-hybrid screen; Yeasts; TCP; Tendril development; Yeast two-hybrid screen; Transcription factor; YABBY; Melon
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-74175-0

Plant tendrils are specialized organs that can twine around other structures to facilitate climbing. They occur in a variety of plant families and have diverse ontogenic origins. In cucurbits, tendrils originate from lateral shoots. Fine mapping verified that the tendril-less ctl mutation of the melon Cucumis melo corresponds to a frameshift mutation in the CmTCP1 gene, which encodes a TCP transcription factor. A yeast two-hybrid screen for CmTCP1/CTL-interacting proteins identified a member of the plant-specific YABBY transcription factor family, which was named CmYAB1. Each of the N- and C-terminal regions of CmTCP1 interacted with CmYAB1. The ctl mutation impaired the interaction between CmTCP1 and CmYAB1. Both proteins interacted in vitro and were localized to the nucleus in plant cells. In situ expression analysis revealed the coexistence of the CmTCP1 and CmYAB1 mRNAs in the abaxial domains of developing tendrils. An RNA-seq analysis of the seven YABBY genes in the melon genome revealed relatively high expression ratios of CmYAB1 in tendrils compared with those in leaves. These results suggest a novel function of the YABBY protein through its interaction with a TCP protein in the development of cucurbit tendrils.