nucleotide sequence of a nodule-specific gene, Nms-25 of Medicago sativa: its primary evolution via exon-shuffling and retrotransposon-mediated DNA rearrangements

We present the primary structure of a nodule-specific gene, Nms-25 from Medicago sativa L. cultivar Nagyszenasi. Analysis of the nucleotide sequence of Nms-25 revealed that this gene shows all the characteristics of an interrupted plant gene consisting of 13 exons and 12 introns. The promoter region...

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Bibliographic Details
Published inPlant molecular biology Vol. 15; no. 2; pp. 295 - 306
Main Authors Vegh, Z, Vincze, E, Kadirov, R, Toth, G, Kiss, G.B
Format Journal Article
LanguageEnglish
Published Netherlands 01.08.1990
Subjects
Amino Acid Sequence
Base Sequence
Biological Evolution
cloning
ddbj/x13287
deletions
DNA - genetics
DNA Transposable Elements
duplication
embl/x13287
evolution
Exons
genbank/x13287
Genes, Plant
genetic recombination
illegitimate recombination
introns
Medicago sativa
Medicago sativa - genetics
Molecular Sequence Data
multigene family
Nitrogen Fixation
nodulin-25
nodulins
nucleotide sequences
Plant Proteins - genetics
promoter regions
restriction mapping
tms1 element
transposons
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Summary:We present the primary structure of a nodule-specific gene, Nms-25 from Medicago sativa L. cultivar Nagyszenasi. Analysis of the nucleotide sequence of Nms-25 revealed that this gene shows all the characteristics of an interrupted plant gene consisting of 13 exons and 12 introns. The promoter region of Nms-25 contains the common promoter elements of plant genes as well as motifs which are supposed to be involved in nodule-specific expression. There are two exon-like sequences in the gene named PE1 and PE2 which are not present in the cDNA clones of Medicago sativa cultivar Cardinal. Intron 9 carries a retrotransposon-like element, Tms1, which might be responsible for downstream deletion events in which a heptanucleotide, ATTAGCT, might have been involved. Most of the exons, exept 1, 12 and 13, are similar to each other both in length (54 bp) and sequence (up to 94% sequence similarity). All exons are interrupted by introns in the same phase (type I). It is suggested that exon-shuffling based on illegitimate recombination in which the ATTAGCT motif might have played an active role, and retrotransposon-mediated DNA rearrangements were the primary events in the molecular evolution of the Nms-25 gene.
ISSN:0167-4412
1573-5028
DOI:10.1007/BF00036915