Erythrosine as a photosensitizer for antimicrobial photodynamic therapy with blue light-emitting diodes – An in vitro study

• Published in: Photodiagnosis and photodynamic therapy Vol. 35; p. 102445
• Main Authors: Gonçalves, Marcela Leticia Leal, Santos, Elaine Marcílio, Renno, Ana Cláudia Muniz, Horliana, Anna Carolina Ratto Tempestini, Cruz, Matheus de Almeida, Parisi, Julia Risso, Prates, Renato Araújo, Leal-Rossi, Adriana, Fernandes, Kristianne Porta Santos, Mesquita-Ferrari, Raquel Agnelli, Bussadori, Sandra Kalil
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.09.2021
• Subjects: Blue Light; Candida albicans; Erythrosine; Photodynamic Therapy; Candida albicans; Blue Light; Erythrosine; Photodynamic Therapy
• ISSN: 1572-1000; 1873-1597
• DOI: 10.1016/j.pdpdt.2021.102445

•A new composition of erythrosine, its pH and cell viability were tested;•Its potencial against C albicans when irratiaded with blue LED for aPDT was tested;•It is a potencial PS, with pH stability, blue light absorbance and cell viability;•It was efficient in aPDT against C. albicans;•Results encourage other studies to check the clinical performance of this PS. This study aims to test the absorbance of a new composition of erythrosine, its pH, cell viability and potential as a photo sensitizer against Candida albicans when irratiaded with blue light emitting-diode (LED). For pH and absorbance tests, erythrosine was prepared at a concentration of 0.03/ml. The cells of the L929 strain were cultured and the alamarBlue® assay was performed on samples to assess cell viability. For the microbiological essay, the strain of Candida albicans ATCC 90028 was selected. Yeast suspensions were divided into the following groups: control without irradiation or photosensitizer (C), irradiated group without photosensitizer (L), photosensitizer group without irradiation (0), and groups that received photosensitizer and irradiation, called aPDT groups. Erythrosine had no significant changes in pH and its absorbance was also consistent (≅400 nm). When it came to cell viability, on the first day, the group that was in contact with the dye and irradiated with the LED in minimun power was found to have the higher cell proliferation. On day 3, both irradiated groups (maximum and minimum) showed the highest cell proliferation. In the microbiological essay with C. albicans, aPDT groups started to show microbial reduction after 60 and 90 s of irradiation and when irradiated for 120 s, 6 microbial reduction logs were found. The erythrosine in question is a PS, with pH stability, blue light absorbance, cell viability and efficacy against C. albicans. More studies with this PS should be encouraged in order to verify its performance in aPDT. This study had the objective of testing the absorbance of a new composition of erythrosine, its pH, cell viability and potencial against Candida albicans when irratiaded with blue light emitting-diode (LED). The erythrosine in question is a potencial photossensitizer, with pH stability, blue/violet light absorbance, cell viability and efficacy against C. albicans, as the survival fractions shows in the graph. [Display omitted]