Role of Insulin-like Growth Factor II Receptor in Transdifferentiation of Free Silica-induced Primary Rat Lung Fibroblasts

• Published in: Biomedical and environmental sciences Vol. 26; no. 12; pp. 979 - 985
• Main Authors: HAO, Chang Fu, LI, Xiao Fang, YAO, Wu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.12.2013; Department of 0ccupational Health and 0ccupational Disease, College of Public Health, Zhengzhou University, Zhengzhou 450001, Henan, China
• Subjects: Animals; Base Sequence; Cell Differentiation - physiology; Cells, Cultured; DNA Primers; Fibroblasts - drug effects; Insulin-like growth factor II receptor; Lung - cytology; Lung - drug effects; Lung fibroblasts; Male; Rats; Rats, Sprague-Dawley; Receptor, IGF Type 2 - genetics; Receptor, IGF Type 2 - physiology; RNA, Messenger - genetics; RT-PCR方法; Silicon Dioxide - pharmacology; Silicosis; Transdifferentiation; 受体; 大鼠; 游离二氧化硅; 肺成纤维细胞; 胰岛素样生长因子; 诱导; 转分化; Lung fibroblasts; Transdifferentiation; Silicosis; Insulin-like growth factor II receptor
• ISSN: 0895-3988; 2214-0190
• DOI: 10.3967/bes2013.033

Objective To study the role of insulin-like growth factor II receptor in free silica-induced transdifferentiation of primary rat lung fibroblasts Methods Rat lung fibroblasts and rat alveolar macrophages were cultured. A transdifferentiation model of primary rat lung fibroblasts was induced by free silica. Levels of a-SMA protein, IGF-liR protein and mRNA were measured by immunocytochemistry, Western blot and RT-PCR, respectively. Lung fibroblasts were treated with Wortmannin. Results The expression levels of a-SMA concentration and decreased after Wortmann and IGF-IIR increased with the increasing free silica n was used. Conclusion The IGF-IIR plays an important role in free silica-induced transdifferentiation of primary rat lung fibroblasts.