Sugar use in relation to shoot induction by sorbitol and cytokinin in apple

Microshoots of 'McIntosh' apple (Malus domestica Borkh.) were grown on Murashige-Skoog (MS) nutrient medium supplemented with either sucrose or sorbitol or with sucrose and an elevated level of cytokinin. Shoot growth was recorded and concentrations of fructose, glucose, sorbitol, and sucr...

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Bibliographic Details
Published inJournal of the American Society for Horticultural Science Vol. 122; no. 4; pp. 476 - 480
Main Author Karhu, S.T
Format Journal Article
LanguageEnglish
Published 01.07.1997
Subjects
6-BENZILADENINA
6-BENZYLADENINE
ALMIDON
AMIDON
APICAL DOMINANCE
BETA-FRUCTOFURANOSIDASE
BIOMASA
BIOMASS
BIOMASSE
BOUTURE
BRANCHING
CARBOHYDRATE METABOLISM
CHEMICAL COMPOSITION
CITOQUININAS
COMPOSICION QUIMICA
COMPOSITION CHIMIQUE
CUTTINGS
CYTOKININE
CYTOKININS
ESQUEJES
FRUCTOFURANOSIDASA
FRUCTOFURANOSIDASE
FRUCTOSA
FRUCTOSE
GLUCOSA
GLUCOSE
GROWTH RATE
INDICE DE CRECIMIENTO
MALUS PUMILA
METABOLISME DES GLUCIDES
METABOLISMO DE CARBOHIDRATOS
MICROPROPAGACION
MICROPROPAGATION
NUTRIENT SOLUTIONS
RAMIFICACION
RAMIFICATION
SACCHAROSE
SOLUCIONES NUTRITIVAS
SOLUTION NUTRITIVE
SORBITOL
STARCH
STEMS
SUCROSA
SUCROSE
TALLO
TAUX DE CROISSANCE
TIGE
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Summary:Microshoots of 'McIntosh' apple (Malus domestica Borkh.) were grown on Murashige-Skoog (MS) nutrient medium supplemented with either sucrose or sorbitol or with sucrose and an elevated level of cytokinin. Shoot growth was recorded and concentrations of fructose, glucose, sorbitol, and sucrose were analyzed in nutrient media and shoots during a 6-week subculture period. Axillary branching was stimulated by high cytokinin and sorbitol media, with increased biomass production and carbohydrate use on the high-BA medium only. The sucrose in the nutrient medium was hydrolyzed to fructose and glucose, which were equally taken up by shoots. Sorbitol was taken up somewhat less effectively. The elevated level of HA decreased sucrose hydrolysis in the nutrient medium. There were high concentrations of sorbitol in shoots grown on the sorbitol medium, and sorbitol also accumulated at the end of the culture period in shoots grown on sucrose. The amount of sucrose was low, and glucose was more abundant than fructose in microshoots. The starch content of leaves was not affected by treatments or sampling time
Bibliography:1997057725
F60
F01
ISSN:0003-1062
2327-9788
DOI:10.21273/JASHS.122.4.476