Monitoring of enzyme catalysed reactions by Fourier transform Raman spectrometry

• Published in: Analytica chimica acta Vol. 370; no. 2; pp. 191 - 197
• Main Authors: Horváth, E, Gajári, J, Kristóf, J, Rédey, Á, Kocsis, L
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 07.09.1998; Elsevier
• Subjects: Analytical chemistry; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Candida antarctica lipase B; Chemistry; Enzyme catalytic reaction; Enzymes and enzyme inhibitors; Exact sciences and technology; FT-Raman spectrometry; Fundamental and applied biological sciences. Psychology; General, instrumentation; Hydrolases; Mandelic acid esterification; Candida antarctica lipase B; Mandelic acid esterification; FT-Raman spectrometry; Enzyme catalytic reaction; Yeast; Enzyme; Triacylglycerol lipase; Esterases; Method; Carboxylic ester hydrolases; Fungi; Enzymatic activity; Candida; Hydrolases; Raman spectrometry; Fungi Imperfecti; Thallophyta
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/S0003-2670(98)00279-7

Esterification of mandelic acid catalysed by Candida antarctica lipase B was studied by Fourier transform (FT) Raman spectrometry in non-aqueous medium. It was found that there is a strict correlation between the intensity of the Raman scattering and the activity of the enzyme. FT-Raman spectrometry seems to be a fast and reliable method for selecting the appropriate enzyme and for determining the optimal enzyme water content. In addition, valuable information can be obtained from the spectra regarding the mechanism of enzyme–substrate bonding.