Quantitative Methods for Metabolite Analysis in Metabolic Engineering

• Published in: Biotechnology and bioprocess engineering Vol. 28; no. 6; pp. 949 - 961
• Main Authors: Ahn, Cheeyoon, Lee, Min-Kyung, Jung, Cheulhee
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Biotechnology and Bioengineering 01.12.2023; Springer Nature B.V; 한국생물공학회
• Subjects: Analytical methods; biochemical pathways; Biotechnology; Chemistry; Chemistry and Materials Science; Chromatography; Decomposition; Efficiency; Engineering; engineers; In vivo methods and tests; Industrial and Production Engineering; Mass spectrometry; Mathematical analysis; Metabolic engineering; Metabolic pathways; Metabolism; Metabolites; Microorganisms; Mutation; Optimization; Particle size; proteins; Quantitative analysis; Review Paper; Scientific imaging; Solvents; 생물공학; metabolite analysis; riboswitch; metabolite-responsive transcription factor; metabolic engineering; throughput
• ISSN: 1226-8372; 1976-3816
• DOI: 10.1007/s12257-022-0200-z

We present a brief overview of metabolic engineering, depicting the necessity of exploiting microorganisms for obtaining desired metabolites and the difficulty of metabolic pathway optimization under numerous conditions. The advantages, limitations, and examples of conventional quantitative analytical methods that focus on accuracy but have low throughput rates are presented. We have also described in vivo analytical methods with high-throughput rates, which indirectly compare the yield of the reporter protein. Additionally, we have explained a few considerations for engineering in vivo analytical methods. This review also highlights the current challenges faced by the analytical methods in the metabolic engineering.