Effect of salicylic acid on the synthesis of ribulose-1,5-bisphosphate carboxylase/oxygenase in barley leaves

• Published in: Journal of plant physiology Vol. 152; no. 4; pp. 381 - 386
• Main Authors: Pancheva, Tania V., Popova, Losanka P.
• Format: Journal Article
• Language: English
• Published: Jena Elsevier GmbH 1998; Elsevier
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; cv. Alfa; Economic plant physiology; Fundamental and applied biological sciences. Psychology; Hordeum vulgare L; Metabolism; Nitrogen metabolism; Nitrogen metabolism and other ones (excepting carbon metabolism); Nutrition. Photosynthesis. Respiration. Metabolism; Plant physiology and development; protein synthesis; RubisCO synthesis; Salicylic acid; Salicylic acid; DTT; protein synthesis; RubisCO; PVP; C i; EDTA; SA; LSU; 14C-AAM; RubisCO synthesis; TCA; SSU; PMSF; Hordeum vulgare L; cv. Alfa; Monocotyledones; Hordeum vulgare; Isotope labelling; Enzyme; Carbon Isotopes; Ribulose-bisphosphate carboxylase; Lyases; Biosynthesis; Plant leaf; Cereal crop; Protein; Carbon-carbon lyases; Gramineae; Carboxy-lyases; Angiospermae; Spermatophyta; Inhibition
• ISSN: 0176-1617; 1618-1328
• DOI: 10.1016/S0176-1617(98)80251-4

Hordeum vulgare L. (cv. Alfa) was grown for 7 days over a range of salicylic acid (SA) concentrations (100 μmol/L-1 mmol/L), and the effect on the level of ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) was examined. The treated plants showed a decrease in the level of total soluble protein, in particular in the level of RubisCO. In the presence of 1 mmol/L SA the level of total soluble protein on a fresh weight basis was about 68% of the control, whereas the level of RubisCO was about 50% that of control plants. The percentage of inhibition on the small subunit was higher, as a result of which the small/large subunit ratio was lower for the experimental variants. When 7-day-old barley seedlings were supplied with SA through the transpiration stream for 24 h no changes in the levels of total soluble protein and RubisCO were observed. The influence of SA on the synthesis of RubisCO was followed by in vivo labelling with a 14C-amino acid mixure ( 14C-AAM). Results demonstrated that SA inhibited both the synthesis of total soluble protein and, more pronounced, the synthesis of RubisCO.