Distribution of secretory inhibitor of platelet microbicidal protein among anaerobic bacteria isolated from stool of children with diarrhea

• Published in: World journal of gastroenterology : WJG Vol. 14; no. 35; pp. 5428 - 5431
• Main Authors: Ivanov, Iuri B, Gritsenko, Viktor A
• Format: Journal Article
• Language: English
• Published: United States The WJG Press and Baishideng 21.09.2008
• Subjects: Actinomyces - isolation & purification; Actinomyces - pathogenicity; Actinomyces - physiology; Bacteria, Anaerobic - isolation & purification; Bacteria, Anaerobic - pathogenicity; Bacteria, Anaerobic - physiology; Bacterial Infections - microbiology; Bacterial Proteins - physiology; Bacteroides fragilis - isolation & purification; Bacteroides fragilis - pathogenicity; Bacteroides fragilis - physiology; beta-Thromboglobulin - antagonists & inhibitors; Bifidobacterium - isolation & purification; Bifidobacterium - pathogenicity; Bifidobacterium - physiology; Child; Diarrhea - microbiology; Eubacterium - isolation & purification; Eubacterium - pathogenicity; Eubacterium - physiology; Feces - microbiology; Humans; Rapid Communication; Virulence - physiology; 分泌抑制剂; 厌氧细菌; 肠道细菌; 血小板
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.14.5428

AIM： To study the secretory inhibitor of platelet microbicidal protein （SIPMP） phenotypes of faecal anaerobic isolates from patients with diarrhea. METHODS： Faecal isolates of anaerobic bacteria （B. fragilis, n = 42; B. longum, n = 70; A. israelii, n = 21; E. lentum, n = 12） from children with diarrhea were tested. SIPMP production was tested by inhibition of platelet microbicidal protein （PMP） bioactivity against B. subtilis and was expressed as percentage of inhibition of PMP bactericidal activity. RESULTS： Among anaerobic isolates 80% of B. longum strains, 85.7% of A. israeli/strains, 50% of E. lentum strains and 92.86% of B. fragilis strains were SIPMP-positive. The isolated anaerobic organisms demonstrated SIPMP production at a mean level of 13.8% ± 0.7%, 14.7% ± 1.8%, 3.9% ± 0.9% （P 〈 0.05） and 26.8% ± 7.5% （P 〈 0.05） for bifidobacteria, A. israelii, E. lentum and B. fragilis, respectively. CONCLUSION： Data from the present study may have significant implications in understanding the pathogenesis of microecological disorders in the intestine, as well as for future improvement in the prevention and therapy of anaerobe-associated infections.