Bovine serum albumin, α-lactoalbumin and β-lactoglobulin partitioning in polyethylene glycol/maltodextrin aqueous-two-phase systems

• Published in: Carbohydrate polymers Vol. 42; no. 3; pp. 279 - 282
• Main Authors: da Silva, LuizaH.M., Meirelles, AntonioJ.A.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.07.2000; Elsevier Science
• Subjects: Animal, plant, fungal and microbial proteins, edible seaweeds and food yeasts; Applied sciences; Aqueous two-phase systems; Biological and medical sciences; Bovine serum albumin; Exact sciences and technology; Food industries; Fundamental and applied biological sciences. Psychology; Natural polymers; Partitioning; Physicochemistry of polymers; Starch and polysaccharides; α-Lactoalbumin; β-Lactoglobulin; Bovine serum albumin; Aqueous two-phase systems; Partitioning; α-Lactoalbumin; β-Lactoglobulin; Ternary system; Partition coefficient; Serum albumin; Molecular weight property relation; Ethylene oxide polymer; Biphasic system; Lactoglobulins; Lactalbumin; Maltodextrin; Animal protein; Physical separation; Oside polymer; Phase partition; Aqueous solution
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/S0144-8617(99)00177-0

The polysaccharide maltodextrin (MD) can provide a low cost alternative to substitute the fractioned dextran for the use with polyethylene glycol (PEG) in aqueous two-phase, two-polymers systems. In this work we have studied the partitioning of Bovine Serum Albumin (BSA), α-Lactoalbumin (α-La) and β-Lactoglobulin (β-Lg) in PEG/MD aqueous two-phase systems. The experiments were performed at local atmospheric pressure (727 mmHg) and 25°C. The influence of the polymers molecular weights and tie-line length were investigated.