High-performance liquid chromatography–electrospray mass spectrometric analysis of pungent constituents of ginger

• Published in: Journal of Chromatography A Vol. 796; no. 2; pp. 327 - 334
• Main Authors: He, Xian-guo, Bernart, Matthew W, Lian, Li-zhi, Lin, Long-ze
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20.02.1998; Elsevier
• Subjects: Aroma and flavouring agent industries; Biological and medical sciences; Food industries; Fundamental and applied biological sciences. Psychology; Gingerol; Shogaol; Shogaol; Ginger; Gingerol; Zingiber officinale; Monocotyledones; Diol; Elution; Gradient; Chemical analysis; Aliphatic compound; Coupled method; Ketol; HPLC chromatography; Diketone; Electrospray; Reversed phase chromatography; Ultraviolet detector; Analysis method; Angiospermae; Benzenic compound; Spermatophyta; Qualitative analysis; Taste; Enone; Mass spectrometry; Zingiberaceae
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(97)01013-3

A gradient elution reversed-phase HPLC separation of ginger extract has been developed, which gives better separation than previous isocratic separations. HPLC–UV–electrospray MS has been successfully utilized to identify the individual pungent constituents in the chromatogram of ginger extract. Seven compounds were positively identified as the major pungent constituents of ginger based on their UV spectra, [M+H] +, [M+Na] + and characteristic sodiated dimer [2M+Na] + ions, and comparison to data for the purified standards: [6]-gingerol and [6]-shogaol. The pungent compounds were assigned as [6]-gingerol, [8]-gingerol, [10]-gingerol, [6]-shogaol, [8]-shogaol, [10]-shogaol and [6]-gingediol. Another eight minor compounds were tentatively identified as gingerol analogues.