Size-relative effectiveness of clove oil as an anaesthetic for rainbow trout (Oncorhynchus mykiss Walbaum, 1792) and goldfish (Carassius auratus Linnaeus, 1758)

• Published in: Acta veterinaria Brno Vol. 79; no. 3; pp. 481 - 490
• Main Authors: Perdikaris, C.,Technological Educational Inst. of Epirus, Igoumenitsa (Greece). Dept. of Aquaculture and Fisheries, Nathanailides, C.,Technological Educational Inst. of Epirus, Igoumenitsa (Greece). Dept. of Aquaculture and Fisheries, Gouva, E.,Technological Educational Inst. of Epirus, Igoumenitsa (Greece). Dept. of Aquaculture and Fisheries, Gabriel, U.U.,Rivers State Univ. of Science and Technology, Port Harcourt (Nigeria). Dept. of Fisheries and Aquatic Environment, Bitchava, K.,University of Thessaly, Karditsa (Greece). Lab. of Ichthyology and Aquatic Animal Medicine, Athanasopoulou, F.,University of Thessaly, Karditsa (Greece). Lab. of Ichthyology and Aquatic Animal Medicine, Paschou, A.,Hatzikosta Hospital, Ioannina (Greece). Haematology and Microbiology Lab, Paschos, I.,Technological Educational Inst. of Epirus, Igoumenitsa (Greece). Dept. of Aquaculture and Fisheries
• Format: Journal Article
• Language: English
• Published: 01.11.2010
• Subjects: ACEITES VEGETALES; ANAESTHESIA; ANAESTHETICS; ANESTESIA; ANESTESICOS; ANESTHESIE; ANESTHESIQUE; ANIMAL WELFARE; AZUCAR EN SANGRE; BIEN-ETRE ANIMAL; BIENESTAR ANIMAL; BLOOD PLASMA; BLOOD SUGAR; CALCIO; CALCIUM; CARASSIUS AURATUS; CLAVO; CLOU DE GIROFLE; CLOVES; Freshwater; GLUCOSA; GLUCOSE; HAEMATOCRIT; HEMATOCRITE; HEMATOCRITO; http://www.fao.org/aos/agrovoc#c_1196; http://www.fao.org/aos/agrovoc#c_14762; http://www.fao.org/aos/agrovoc#c_16101; http://www.fao.org/aos/agrovoc#c_23597; http://www.fao.org/aos/agrovoc#c_3287; http://www.fao.org/aos/agrovoc#c_33779; http://www.fao.org/aos/agrovoc#c_34843; http://www.fao.org/aos/agrovoc#c_35395; http://www.fao.org/aos/agrovoc#c_381; http://www.fao.org/aos/agrovoc#c_443; http://www.fao.org/aos/agrovoc#c_6139; http://www.fao.org/aos/agrovoc#c_7778; http://www.fao.org/aos/agrovoc#c_8170; http://www.fao.org/aos/agrovoc#c_9320; http://www.fao.org/aos/agrovoc#c_9324; HUILE VEGETALE; ONCORHYNCHUS MYKISS; PLANT OILS; PLASMA SANGUIN; PLASMA SANGUINEO; POTASIO; POTASSIUM; SUCRE DU SANG; SYZYGIUM AROMATICUM; TEMPS; TIEMPO; TIME; Czech Rep., Jihhomoravsky, Brno
• ISSN: 0001-7213; 1801-7576
• DOI: 10.2754/avb201079030481

The purpose of this work was to investigate the size-relative effectiveness of clove oil as an anaesthetic for rainbow trout and goldfish. In total, 128 rainbow trout (two groups of 20-23 and 30-33 cm mean fork length) and 160 goldfish (four size groups of 1.5-2.5, 5-7, 11-15 and 20-25 cm) were anaesthetized at different clove oil concentrations of 50, 100, 150 mg/L for trouts and 75, 100, 150 mg/L for goldfish. Rainbow trout exhibited total loss of balance and no response to external stimuli with shorter induction time as dosage increased (120.5 s, 64.4 s and 44.3 s, respectively). Goldfish exhibited total loss of balance and no response to external stimuli after induction time that varied with dosage used and body size of fish. The small fish (1.5-7 cm) exhibited shorter induction time which ranged from 84.28 s at 75 mg/L clove oil to 41.14 s at 150 mg/L clove oil. The larger fish had a longer induction time inversely related to the dosage. Recovery time was longer than induction time in both species. Both species recovered within 6 min after anaesthesia at 150 mg/L clove oil. Clove oil did not produce marked changes (P less than 0.05) in the physiological indicators of goldfish compared to the control. However, marked changes (P less than 0.05) were exhibited in the haematocrit of treated rainbow trout that also exhibited non-significant hyperkalaemia and hyperglycaemia. For both fish species, clove oil was effective, producing minimum stress and zero mortalities, and can be recommended as an effective anaesthetic.