Determination of saikosaponins by micellar electrokinetic capillary chromatography

In this study, we examine the feasibility of determining saikosaponins a, b1, b2, c and d, i.e., the bioactive components in Bupleuri Radix, using the micellar electrokinetic capillary chromatography technique. Results obtained from using different anion surfactants, bile salt or sodium dodecyl sulf...

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Bibliographic Details
Published inJournal of Chromatography A Vol. 759; no. 1; pp. 193 - 201
Main Authors Hsieh, You-Zung, Huang, Hsi-Ya
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 24.01.1997
Elsevier
Subjects
Biological and medical sciences
Buffer composition
General pharmacology
Medical sciences
Pharmaceutical analysis
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Saikosaponins
Saponins
Asia
China
Saponins
Pharmaceutical analysis
Saikosaponins
Buffer composition
Pentacyclic compound
Chemical analysis
Root
Pharmacognosy
Micellar electrokinetic capillary chromatography
Extract
Mobile phase
Disaccharide
Medicinal plant
Saponin
Cyclodextrin
Chemical modification
Folk medicine
Plant origin
Triterpene
Isolation
Chemical composition
Glycoside
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Summary:In this study, we examine the feasibility of determining saikosaponins a, b1, b2, c and d, i.e., the bioactive components in Bupleuri Radix, using the micellar electrokinetic capillary chromatography technique. Results obtained from using different anion surfactants, bile salt or sodium dodecyl sulfate (SDS), are also compared. By using bile salt as a pseudostationary phase, the baseline separation of saikosaponins is observed; however, the detection limit is unfavorable. Whereas the baseline resolution of saikosaponins is achieved after adding γ-cyclodextrin in the SDS running buffer. In addition, another separating method is developed with the mixed micellar system of SDS and Brij 35 containing γ-cyclodextrin. The contents of saikosaponins in traditional Chinese medicinal prescriptions, such as Sheau Chair Hwu Tang, Bupleuri Radix plant and Chair Hwu San are analyzed by one of the above methods. The entire separation can be completed within 9 min by these methods. The R.S.D. values of the migration times are all below 1%. Moreover, the detection limits of saikosaponins vary from 7 to 33 μg/ml.
ISSN:0021-9673
DOI:10.1016/S0021-9673(96)00768-6