Automation of DNA Sequencing: A System to Perform the Sanger Dideoxysequencing Reactions

The determination of the complete nucleotide sequence of large DNA molecules is a tedious and time-consuming process. The repetitive nature of the task makes it an ideal subject for automation. We report here a system designed and constructed to perform the chain termination reactions of the Sanger...

Full description

Saved in:
Bibliographic Details
Published inBio/Technology Vol. 3; no. 10; pp. 911 - 915
Main Authors Martin, William J., Warmington, John R., Galinski, Bohdan R., Gallagher, Michael, Davies, R. Wayne, Beck, Maurice S., Oliver, Stephen G.
Format Journal Article
LanguageEnglish
Published New York Nature Publishing Group US 01.10.1985
Subjects
Agriculture
Bioinformatics
Biomedical and Life Sciences
Biomedical Engineering/Biotechnology
Biomedicine
Biotechnology
Life Sciences
research-paper
Online AccessGet full text

Cover

More Information
Summary:The determination of the complete nucleotide sequence of large DNA molecules is a tedious and time-consuming process. The repetitive nature of the task makes it an ideal subject for automation. We report here a system designed and constructed to perform the chain termination reactions of the Sanger sequencing procedure automatically. It is capable of carrying out these reactions for from five to twelve DNA templates at one time. The system takes 12 minutes to dispense 12 templates, add the reagents and mix them; the complete cycle time, including incubations, is 45 minutes.
ISSN:0733-222X
1546-1696
DOI:10.1038/nbt1085-911