Curcumin attenuates inflammatory response in IL-1β-induced human synovial fibroblasts and collagen-induced arthritis in mouse model

• Published in: International immunopharmacology Vol. 10; no. 5; pp. 605 - 610
• Main Authors: Moon, Dong-Oh, Kim, Mun-Ok, Choi, Yung Hyun, Park, Yung-Min, Kim, Gi-Young
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier B.V 01.05.2010; Elsevier
• Subjects: Biological and medical sciences; Collagen-induced arthritis; Curcumin; Cyclooxygenase-2; Diseases of the osteoarticular system; Inflammatory joint diseases; Matrix metalloproteinase; Medical sciences; Nuclear factor-κB; Pharmacology. Drug treatments; Prostaglandin E 2; Curcumin; Cyclooxygenase-2; Matrix metalloproteinase; Nuclear factor-κB; Prostaglandin E 2; Collagen-induced arthritis; Animal model; Cyclooxygenase 2; Diseases of the osteoarticular system; Prostaglandin E2; Autoimmune disease; Metalloendopeptidases; Inflammatory joint disease; Polyphenol; Synovial membrane; Eicosanoid; Transcription factor NFκB; Prostaglandin E; Human; Immunopathology; Enzyme; Tyrosine kinase inhibitor; Arachidonic acid derivatives; Cytokine; Rodentia; Interleukin 1β; Inflammation; Peptidases; Vertebrata; Chronic; Mammalia; Mouse; Rheumatoid arthritis; Phenols; Hydrolases; Oxidoreductases; Collagen induced arthritis; Fibroblast
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2010.02.011

Curcumin, a major component of turmeric, has been shown to exhibit anti-oxidant and anti-inflammatory activities. The present study was performed to determine whether curcumin is efficacious against both collagen-induced arthritis (CIA) in mice and IL-1β-induced activation in fibroblast-like synoviocytes (FLSs). DBA/1 mice were immunized with bovine type II collagen (CII) and treated with curcumin every other day for 2 weeks after the initial immunization. For arthritis, we evaluated the incidence of disease and used an arthritis index based on paw thickness. In vitro proliferation of CII- or concanavalin A-induced splenic T cells was examined using IFN-γ production. Pro-inflammatory cytokines TNF-α and IL-1β were examined in the mouse ankle joint and serum IgG1 and IgG2a isotypes were analyzed. The expression levels of prostaglandin E 2 (PGE 2), cyclooxygenase-2 (COX-2), and matrix metalloproteinases (MMPs) in human FLSs were also determined. The results showed that compared with untreated CIA mice, curcumin-treated mice downregulated clinical arthritis score, the proliferation of splenic T cells, expression levels of TNF-α and IL-1β in the ankle joint, and expression levels of IgG2a in serum. Additionally, by altering nuclear factor (NF)-κB transcription activity in FLSs, curcumin inhibited PGE 2 production, COX-2 expression, and MMP secretion. These results suggest that curcumin can effectively suppress inflammatory response by inhibiting pro-inflammatory mediators and regulating humoral and cellular immune responses.