Assemble of poly(aniline-co- o-aminobenzenesulfonic acid) three-dimensional tubal net-works onto ITO electrode and its application for the direct electrochemistry and electrocatalytic behavior of cytochrome c

• Published in: Electrochemistry communications Vol. 8; no. 7; pp. 1137 - 1141
• Main Authors: Jiang, Xiue, Zhang, Lei, Dong, Shaojun
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.07.2006; Amsterdam Elsevier Science; New York, NY
• Subjects: Biological and medical sciences; Cytochrome c; Direct electrochemistry; Electrochemistry; Fundamental and applied biological sciences. Psychology; Hydrogen peroxide; Molecular biophysics; Physical chemistry in biology; Sulfonated conducting polymer nanotubes; Cytochrome c; Hydrogen peroxide; Sulfonated conducting polymer nanotubes; Direct electrochemistry; Catalytic reaction; Indium tin oxide electrode; Aromatic polymer; Electron transfer; Tubular electrode; Conducting polymers; Chemical reduction; Cyclic voltammetry; Electrocatalysis; Aniline copolymer; Electrode reaction; Reaction mechanism; Electrochemical reaction; Catalyst activity; Modified material
• ISSN: 1388-2481; 1873-1902
• DOI: 10.1016/j.elecom.2006.05.004

Stable electroactive film of poly(aniline-co– o-aminobenzenesulfonic acid) three-dimensional tubal net-works was assembled on indium oxide glass (ITO) successfully, and the cytochrome c was immobilized on the matrix by the electrostatic interactions. The adsorbed cytochrome c showed a good electrochemical activity with a pair of well-defined redox waves in pH 6.2 phosphate buffer solution, and the adsorbed protein showed more faster electron transfer rate (12.9 s −1) on the net-works matrix than those of on inorganic porous or even nano-materials reported recently. The immobilized cytochrome c exhibited a good electrocatalytic activity and amperometric response (2 s) for the reduction of hydrogen peroxide (H 2O 2). The detection limit for H 2O 2 was 1.5 μM, and the linear range was from 3 μM to 1 mM. Poly(aniline-co- o-aminobenzenesulfonic acid) three-dimensional tubal net-works was proved to be a good matrix for protein immobilization and biosensor preparation.