The cytotoxic activity of two D-galactose-binding lectins purified from marine invertebrates
The present investigation was undertaken in order to evaluate the cytotoxic effect of two D-galactose-binding lectins using the brine shrimp lethality bioassay technique. Both lectins were purified from the marine invertebrates, sea hare Aplysia kurodai eggs and polychaete Perineries nuntia by conve...
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Published in | Archives of biological sciences Vol. 62; no. 4; pp. 1027 - 1034 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
2010
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Online Access | Get full text |
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Summary: | The present investigation was undertaken in order to evaluate the cytotoxic
effect of two D-galactose-binding lectins using the brine shrimp lethality
bioassay technique. Both lectins were purified from the marine invertebrates,
sea hare Aplysia kurodai eggs and polychaete Perineries nuntia by
conventional affinity chromatography methods. The molecular mass of Aplysia
kurodai egg lectin (AKL) was determined to be 32 kDa and 56 kDa by sodium
dodecyl sulfate polyacrylamide gel electrophoresis under reducing and
non-reducing conditions, respectively. On the other hand, polychaete
Perineries nuntia lectin (PnL) was determined to be 32 kDa in both reducing
and non-reducing conditions. AKL and PnL showed strong agglutination activity
against trypsinized and glutaraldehyde-fixed human and rabbit erythrocytes.
AKL significantly affects the mortality rate of brine shrimp. Experimental
results revealed that AKL was found to be more toxic (63.33% mortality) than
PnL (33.33% mortality) and the mortality rate of brine shrimp nauplii was
increased with the increase in concentration of lectins. These cytotoxic
results indicate that future findings of lectin applications obtained from
marine invertebrates may be of importance to clinical microbiology, and that
they could have application as potent chemotherapeutic agents.
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ISSN: | 0354-4664 1821-4339 |
DOI: | 10.2298/ABS1004027K |