Plasma Cysteine/Cystine Reduction Potential Correlates with Plasma Creatinine Levels in Chronic Kidney Disease

Background/Aims: Oxidative stress has been considered a nontraditional risk factor for cardiovascular disease in the chronic kidney disease (CKD) population, possibly triggered by uremic toxicity. Methods: A chromatographic method with coulometric detection was adapted to directly and simultaneously...

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Bibliographic Details
Published inBlood purification Vol. 34; no. 3-4; pp. 231 - 237
Main Authors Rodrigues, Silvia D., Batista, Giuseppe B., Ingberman, Max, Pecoits-Filho, Roberto, Nakao, Lia S.
Format Journal Article
LanguageEnglish
Published Basel, Switzerland Karger 01.01.2012
Subjects
Adolescent
Adult
Aged
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Biological and medical sciences
Biomarkers - blood
Chromatography, High Pressure Liquid
Creatinine - blood
Cysteine - blood
Cystine - blood
Emergency and intensive care: renal failure. Dialysis management
Female
Humans
Intensive care medicine
Male
Medical sciences
Middle Aged
Original Paper
Oxidative Stress
Renal Insufficiency, Chronic - blood
Young Adult
Oxidative stress
Cysteine
Reduction potential
Chronic kidney disease
Uremia
Sulfur containing aminoacid
Creatinine
Kidney disease
Thiol
Urinary system disease
Renal failure
Chronic kidney diseases
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Summary:Background/Aims: Oxidative stress has been considered a nontraditional risk factor for cardiovascular disease in the chronic kidney disease (CKD) population, possibly triggered by uremic toxicity. Methods: A chromatographic method with coulometric detection was adapted to directly and simultaneously determine cysteine (Cys) and cystine (Cyss) in plasma samples. Healthy subjects and CKD subjects in different stages were analyzed. The free Cys and free Cyss levels in their plasma were determined, and the reduction potential [E h(Cyss/2Cys) ] was calculated with the Nernst equation. Results: Healthy plasma presented E h(Cyss/2Cys) of –123 ± 7 mV. Plasma E h(Cyss/2Cys) correlated significantly with creatinine levels (p < 0.0001, r = 0.62). Conclusion: Plasma E h(Cyss/2Cys) correlated with increased levels of plasma creatinine, supporting the view that uremia triggers oxidative stress. In addition, it may be used as a quantitative oxidative stress biomarker in uremic conditions.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0253-5068
1421-9735
DOI:10.1159/000342627