Structural analysis of the promoter of tomato 1-aminocyclopropane-1-carboxylate synthase 6 gene (Le-ACS6)

• Published in: Chinese science bulletin Vol. 52; no. 9; pp. 1217 - 1222
• Main Authors: Lin, JingYu, Fan, Rong, Wan, XiaoRong, Charng, Yee-yung, Wang, NingNing
• Format: Journal Article
• Language: English
• Published: Beijing Springer Nature B.V 01.05.2007; Department of Plant Biology and Ecology, Nankai University, Tianjin 300071, China%Institute of BioAgricutural Sciences, Academia Sinica, Nankang, Taipei 11529, China
• Subjects: 1-Aminocyclopropane-1-carboxylate synthase; Biosynthesis; Ethylene; Feedback; Feedback control; Fruits; Fusion protein; Gene deletion; Genetic transformation; Lycopersicon esculentum; Negative feedback; Nucleotides; Particle bombardment; Pericarp; Reporter gene; Ripening; Structural analysis; Tomatoes; Le-ACS6 oromoter; cis-acting element; ethylene; Micro-Tom
• ISSN: 1001-6538; 2095-9273; 1861-9541; 2095-9281
• DOI: 10.1007/s11434-007-0183-3

Ethylene plays an important role in the regulation of many growth and developmental processes of higher plants. In tomato, Le-ACS6, a member of the ACC synthase multigene family involved in system 1 ethylene biosynthesis during fruit ripening, is subject to negative feedback regulation by ethylene. To identify the cis-elements that are responsible for the negative feedback control, we established an in vitro transient assay system employing particle bombardment on mature-green tomato fruit pericarp to examine the expression of a luciferase (LUC) reporter gene driven by a 5′-serially deleted Le-ACS6 promoter. The results localized putative cis-elements required for negative ethylene-response between −347 and −266 upstream from the translational start site ATG. Several lines of stable transformation of the Le-ACS6 promoter and GUS reporter fusion gene containing internal deletion from −347 to −266 were generated. The expression pattern of the GUS reporter showed that removal of the nucleotides from −347 to −266 completely eliminated the response of the Le-ACS6 promoter to exogenous ethylene.