Förster resonance energy transfer between acridinediones and selected fluorophores—Medium dependence

• Published in: Journal of luminescence Vol. 138; pp. 242 - 250
• Main Authors: Krishnaveni, R., Ramamurthy, P.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.06.2013; Elsevier
• Subjects: Acridinedione; Acridinedione modified β-CD; Condensed matter: electronic structure, electrical, magnetic, and optical properties; Energy transfer; Exact sciences and technology; Fluorescence; Optical properties and condensed-matter spectroscopy and other interactions of matter with particles and radiation; Photoluminescence; Physics; Resonance; Safranine; Fluorescence; Safranine; Acridinedione modified β-CD; Acridinedione; Resonance; Energy transfer; Cadmium; Dipole dipole interaction; Organic dye; Acridinedione modified p-CD; Time resolved spectra; Lifetime; Polymer solutions; Acceptor center; Donor center
• ISSN: 0022-2313; 1872-7883
• DOI: 10.1016/j.jlumin.2013.01.025

We report highly efficient Förster resonance energy transfer process between acridinedione dyes and basic fluorophores. FRET between free and β-cyclodextrin modified acridinediones as donors and fluorophoric dyes like safranine as acceptor were investigated in an alcoholic medium, polymer solution and a polymeric film. Efficiency of the processes were experimentally found by steady-state and time-resolved experiments for different donor and acceptor combinations. The associated spectral parameters viz., R0, J(λ) were calculated, the Stern–Volmer relations based on fluorescence intensity and lifetime were constructed and the rates of energy transfer were calculated. The results indicated that the dominant mechanism responsible for the excitation energy transfer is that of resonance transfer due to long range dipole–dipole interaction and the process was found to be highly efficient when the medium was a constrained one as in the case of a polymeric film. A multifold enhancement in efficiency of energy transfer was also observed when the donor was a modified acridinedione when compared to a free acridinedione. The reason is attributed to the effective binding of the acceptor into the cavity of β-cyclodextrin. [Display omitted] ► FRET between acridinediones and fluorophoric acceptors were investigated. ► FRET analysis was carried out in methanol, a 5% PVA solution and in PVA matrix. ► FRET efficiency was maximum when the medium was PVA matrix. ► FRET efficiency was also more when β-CD modified ADR is used as the donor. ► Efficiency enhancement is due to the inclusion of acceptor into the cavity of β-CD.