Effect of the luminol signal enhancer on the chemiluminescence intensity and kinetics

• Published in: Journal of luminescence Vol. 132; no. 4; pp. 1021 - 1024
• Main Authors: Yan-li, Liang, Fei, Yu, Song-cheng, Yu, Yong-jun, Wu, Hong-quan, Zhang, Ling-bo, Qu
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.04.2012; Elsevier
• Subjects: Analytical biochemistry: general aspects, technics, instrumentation; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chemiluminescence; Fundamental and applied biological sciences. Psychology; Luminol; Signal enhancer; Chemiluminescence; Signal enhancer; Luminol; Line intensity; Phenols; Kinetics; Measurement method; Catalyst
• ISSN: 0022-2313; 1872-7883
• DOI: 10.1016/j.jlumin.2011.11.029

The novel p-phenol derivatives, 4-(1-imidazolyl)-phenol, 4-hydroxybiphenyl, 4-hydroxy-4′-iodobiphenyl were employed as highly potent signal enhancers of luminol-hydrogen peroxide (H 2O 2)-horseradish peroxidase (HRP) chemiluminescence (CL) system. The CL reaction conditions were optimized, and the enhancement characteristics of these enhancers were compared with each other. The employment of these molecules greatly affected important assay parameters. Practically, the use of a novel enhancer, even a slightly change of the structure (or concentration) of 4-substituted phenol derivative, could affect assay properties quite dramatically. Furthermore, the use of different enhancers in the luminol–H 2O 2–HRP system can affect not only the intensity of the CL signal, which is well known, but also its kinetics. The experiment data indicated that the stronger intensity was combined with a more rapid decrease of the CL signal. ► 4-IMP, 4-BIP and HIOP take on different signal enhancement and light kinetics. ► The employment of 4-BIP resulted in a significant improvement of the detection limit. ► The magnitude of the signal enhancement was about 2.5-fold in the same system.