Polyunsaturated fatty acid composition and lipid metabolism in cultured cardiomyocyte and isolated working rat heart

• Published in: Nutrition research (New York, N.Y.) Vol. 13; no. 9; pp. 1003 - 1015
• Main Authors: Demaison, L., Bouveret, P., Grynberg, A.
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.09.1993; Elsevier Science
• Subjects: Biological and medical sciences; Cardiac lipid metabolism; Fundamental and applied biological sciences. Psychology; Heart; Polyunsaturated fatty acids; Vertebrates: cardiovascular system; Polyunsaturated fatty acids; Cardiac lipid metabolism; Heart; Nutrition; Rat; Rodentia; Contractility; Polyunsaturated fatty acid; Phospholipid; Lipids; Metabolism; Feeding; Myocyte; Vertebrata; Mammalia; Diet; Chemical composition; Circulatory system
• ISSN: 0271-5317; 1879-0739
• DOI: 10.1016/S0271-5317(05)80520-2

The influence of the phospholipid polyunsaturated fatty acid (PUFA) profile on fatty acid metabolism was investigated in cultured cardiomyocytes and isolated working rat hearts. Alteration of the n-6/n-3 PUFA ratio of membrane phospholipids in the whole heart was achieved by feeding the rats a diet containing either sunflower seed oil or linseed oil. Cardiomyocytes were incubated in a medium containing either C18:2n-6 and C20:4n-6 or C18:3n-3 and C20:5n-3. The lipid metabolism was studied in the perfused heart by injection of a bolus of 1- 14C palmitate and further perfusion with non radioactive medium for different periods (0.5 to 5 minutes). Cardiac work and heart rate were monitored throughout the experiment. The cultured cardiomyocytes were incubated for different times (30 to 120 minutes) in a medium containing 1- 14C palmitate. The n-3 PUFA rich treatment provoked a large decrease in the n-6/n-3 ratio of membrane phospholipids that was characterized by an accumulation of C22:6n-3 in the whole heart and shorter chain n-3 PUFA in the cultured cells. In the heart, palmitate oxidation was not affected by the changes in PUFA profile but its incorporation into phospholipids was increased in the n-3 rich group. Neither cardiac work nor heart rate were significantly affected. The n-3 and n-6 rich cardiomyocytes exhibited a similar rate of palmitate oxidation. Thus, the linseed oil-induced changes in the phospholipid PUFA profile of the myocardium did not modify the contractile function nor the fatty acid oxidation.