Sucrose rinse modulates the salivary behavior of carbonic anhydrase VI and its buffering capacity: a longitudinal study in 4 to 6.5-year-old children

• Published in: PeerJ (San Francisco, CA) Vol. 12; p. e17429
• Main Authors: de Souza, Thayse Rodrigues, Zancope, Bruna Raquel, de Sousa, Emerson Tavares, Parisotto, Thais Manzano, Rocha Marques, Marcelo, Nobre Dos Santos, Marinês
• Format: Journal Article
• Language: English
• Published: United States PeerJ Inc 30.05.2024
• Subjects: Biochemistry; Buffers; Carbonic Anhydrases; Carbonic Anhydrases - metabolism; Caries; Child; Child, Preschool; Children; Dental Caries; Dentistry; Female; Humans; Hydrogen-Ion Concentration; Longitudinal Studies; Male; Mouthwashes; Pediatrics; Saliva - chemistry; Saliva - enzymology; Sucrose - metabolism; Caries; Carbonic Anhydrases; Children
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.17429

Carbonic anhydrase VI (CA VI) is crucial in regulating oral pH and predicting susceptibility to dental caries. The hypothesis posits that caries activity may alter the CA VI function, diminishing its capacity to regulate pH effectively and potentially exacerbating cariogenic challenges. This 1-year cohort study sought to investigate the enzymatic activity of salivary CA VI and buffering capacity following a 20% sucrose rinse in 4 to 6.5-year-old children. This research involved 46 volunteers categorized into three groups based on their caries status after follow-up: caries-free (C ), arrested caries (C ), and caries active (C ). Children underwent visible biofilm examination and saliva collection for salivary flow rate, buffering capacity, and CA VI analyses before and after a 20% sucrose rinse. A reduction in the buffering capacity was observed after sucrose rinse in all groups. The CA VI activity decreased significantly in C and C groups after sucrose rinse, although it did not change in the C group. An improvement in the buffering capacity and salivary flow rate was found at follow-up when compared with the baseline. After 1-year follow-up, buffering capacity and salivary flow rate increased in all groups, whilst the CA VI activity reduced only in C and C children. Sucrose rinse universally reduces the salivary buffering capacity, while caries activity may disrupt CA VI activity response during a cariogenic challenge. After a year, increased salivary flow enhances buffering capacity but not CA VI activity in caries-active children.