Molecularly imprinted polymers by suspension polymerisation in perfluorocarbon liquids, with emphasis on the influence of the porogenic solvent

• Published in: Journal of Chromatography A Vol. 787; no. 1; pp. 55 - 66
• Main Authors: Ansell, Richard J., Mosbach, Klaus
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 07.11.1997; Elsevier
• Subjects: Analytical chemistry; Applied sciences; Biological properties; Chemistry; Chromatographic methods and physical methods associated with chromatography; Enantiomer separation; Exact sciences and technology; Molecular imprinting; Organic polymers; Other chromatographic methods; Physicochemistry of polymers; Porogenic solvents; Properties and characterization; Stationary phases, LC; Molecular imprinting; Stationary phases, LC; Enantiomer separation; Porogenic solvents; Particle size; Separation; HPLC chromatography; Experimental study; Property processing relationship; Crosslinked copolymer; Methacrylic acid copolymer; α-Aminoacid; Pore size; Ultraviolet detector; Optical resolution; Template polymerization; Enantiomer; Preparation; Methacrylate copolymer; Stationary phase
• DOI: 10.1016/S0021-9673(97)00643-2
• ISSN: 0021-9673

Methacrylic acid (MAA)-trimethylolpropane trimethacryl ate (TRIM) copolymer beads imprinted with tert.-butoxy-carbonyl- l-phenylalanine (Boc- l-Phe) have been prepared by suspension polymerisation in a perfluorocarbon liquid. The suspension polymerisation procedure has been adapted to work with a number of different porogenic solvents, and with UV initiated or thermal initiated polymerisation. The resulting polymers have been evaluated as stationary phases for the chromatographic separation of Boc- d,l-Phe. Polymer beads of average diameter 8–25 μm packed into 100×4.6 mm chromatography columns were used with a mobile phase of dichloromethane-acetic acid (99:1, v/v) at flow-rates up to 5 ml min −1. A polymer prepared with 1,2-dichloroethane as porogenic solvent (average bead diameter 15.1 μm) was capable of the baseline separation of 100 μg Boc- d,l-Phe at a flow-rate of 0.5 ml min −1.