Molecularly imprinted polymers by suspension polymerisation in perfluorocarbon liquids, with emphasis on the influence of the porogenic solvent
Methacrylic acid (MAA)-trimethylolpropane trimethacryl ate (TRIM) copolymer beads imprinted with tert.-butoxy-carbonyl- l-phenylalanine (Boc- l-Phe) have been prepared by suspension polymerisation in a perfluorocarbon liquid. The suspension polymerisation procedure has been adapted to work with a nu...
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Published in | Journal of Chromatography A Vol. 787; no. 1; pp. 55 - 66 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
Amsterdam
Elsevier B.V
07.11.1997
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Methacrylic acid (MAA)-trimethylolpropane trimethacryl ate (TRIM) copolymer beads imprinted with
tert.-butoxy-carbonyl-
l-phenylalanine (Boc-
l-Phe) have been prepared by suspension polymerisation in a perfluorocarbon liquid. The suspension polymerisation procedure has been adapted to work with a number of different porogenic solvents, and with UV initiated or thermal initiated polymerisation. The resulting polymers have been evaluated as stationary phases for the chromatographic separation of Boc-
d,l-Phe. Polymer beads of average diameter 8–25 μm packed into 100×4.6 mm chromatography columns were used with a mobile phase of dichloromethane-acetic acid (99:1, v/v) at flow-rates up to 5 ml min
−1. A polymer prepared with 1,2-dichloroethane as porogenic solvent (average bead diameter 15.1 μm) was capable of the baseline separation of 100 μg Boc-
d,l-Phe at a flow-rate of 0.5 ml min
−1. |
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ISSN: | 0021-9673 |
DOI: | 10.1016/S0021-9673(97)00643-2 |