Expression of tubulin folding cofactor B in mouse hepatic ischemia-reperfusion injury

• Published in: Biomedical reports Vol. 6; no. 5; pp. 525 - 531
• Main Authors: Gong, Jianhua, Wang, Junyi, Tian, Yu, Zhang, Jing, Liang, Wenjin, Li, Zeming, Yu, Jidong, Tang, Bo, He, Songqing
• Format: Journal Article
• Language: English
• Published: England Spandidos Publications 01.05.2017; D.A. Spandidos
• Subjects: Care and treatment; Cellular proteins; Development and progression; Gene expression; Genetic aspects; Health aspects; Reperfusion injury; ischemia-reperfusion injury; tubulin folding cofactor B; cell microtubule; cytoskeleton
• ISSN: 2049-9434; 2049-9442
• DOI: 10.3892/br.2017.891

The aim of the present study was to investigate the association between tubulin folding cofactor B (TBCB) expression and ischemia-reperfusion injury (IRI) in mice. A total of 48 C57BL/6 mice were randomly divided into a control group (Sham, n=6) and an ischemia-reperfusion group (n=42). The ischemia-reperfusion group was further divided into 6 subgroups as per different times after reperfusion (2, 4, 6, 8, 12 and 24 h), with 7 mice per subgroup. A hepatic IRI model was established in mice by clamping the hepatic hilum. Morphology, serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), interleukin 6 (IL-6) and tumor necrosis factor-α (TNF-α), and the expression level of TBCB were detected. Compared with the control group, the livers from the ischemia-reperfusion group were significantly changed, particularly at 12 h following ischemia-reperfusion, with obvious hepatic cell degeneration and necrosis. The ALT, AST, IL-6 and TNF-α levels in the sera of the mice in the hepatic ischemia-reperfusion group were increased at all time points following ischemia-reperfusion, and were the highest at 12 h, demonstrating statistically significant differences when compared with the control group (P<0.05). Furthermore, the expression levels of TBCB, TNF-α and IL-6 were significantly increased at all time-points following ischemia-reperfusion, and were the most significant at 12 h. At 24 h following ischemia-reperfusion, the expression levels had decreased. The present study indicated that TBCB expression is associated with TNF-α and IL-6 expression levels in mice with hepatic ischemia-reperfusion, and may be key in the development of liver injury during ischemia-reperfusion in mice.