Preparation of hydrophobic and hydrophilic albumin microspheres and determination of surface carboxylic acid and amino residues

Albumin microspheres (MS) have been studied extensively as delivery systems for targeting drugs since they are biodegradable, non-toxic, relatively easy to prepare and their size range can be controlled. A method for albumin MS production was developed which was faster, processed larger quantities o...

Full description

Saved in:
Bibliographic Details
Published inInternational journal of pharmaceutics Vol. 151; no. 1; pp. 47 - 55
Main Authors MacAdam, A.B., Shafi, Z.B., James, S.L., Marriott, C., Martin, G.P.
Format Journal Article
LanguageEnglish
Published Amsterdam Elsevier B.V 12.05.1997
Elsevier
Subjects
14C-Glycine ethyl ester hydrochloride: 14C-Sodium acetate
Albumin microspheres
Biological and medical sciences
Carboxymethylated
Crosslinking
General pharmacology
Glutaraldehyde
Hydrophilic
Hydrophobic
Medical sciences
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
Surface amino residues
Surface carboxylic acid residues
Hydrophilic
Glutaraldehyde
14C-Glycine ethyl ester hydrochloride: 14C-Sodium acetate
Carboxymethylated
Crosslinking
Surface carboxylic acid residues
Albumin microspheres
Surface amino residues
Hydrophobic
Proteins
Microsphere
Pharmaceutical technology
Molecular structure
Manufacturing process
Formulation
Albumin
Drug carrier
Property structure relationship
Hydrophobicity
Hydrophily
Online AccessGet full text

Cover

More Information
Summary:Albumin microspheres (MS) have been studied extensively as delivery systems for targeting drugs since they are biodegradable, non-toxic, relatively easy to prepare and their size range can be controlled. A method for albumin MS production was developed which was faster, processed larger quantities of starting material than previous methods, and had chemically reactive groupings on the MS surface to which ligands could be attached. Relatively hydrophobic, hydrophilic and also carboxymethylated MS were manufactured. The number of carboxylic acid residues was determined on the surface of these MS using14C-glycine ethyl ester hydrochloride as a probe, and the number of amino groups was determined using 14C-sodium acetate as a probe. The number of carboxylic acid residues per unit surface area for the hydrophobic, hydrophilic and carboxymethylated MS was 2.1 × 104, 4.1 × 104 and 8.4 × 104, respectively, and the number of amino acid residues was 2.2 × 103, 5.0 × 102 and 5.0 × 102, respectively
ISSN:0378-5173
1873-3476
DOI:10.1016/S0378-5173(97)04886-2