Localization of the human growth arrest-specific gene (GAS1) to chromosome bands 9q21.3-q22, a region frequently deleted in myeloid malignancies

• Published in: Genomics (San Diego, Calif.) Vol. 18; no. 3; pp. 731 - 733
• Main Authors: Evdokiou, Andreas, Webb, Graham C., Peters, Gregory B., Dobrovic, Alexander, O'Keefe, Denise S., Forbes, Ian J., Cowled, Prudence A.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.12.1993; Elsevier
• Subjects: 3T3 Cells; Animals; Base Sequence; Biological and medical sciences; chromosome 9; Chromosome Banding; Chromosome Deletion; Chromosomes, Human, Pair 9; Classical genetics, quantitative genetics, hybrids; Female; Fundamental and applied biological sciences. Psychology; GAS-1 gene; gene mapping; genes; Genetics of eukaryotes. Biological and molecular evolution; growth arrest-specific gene; Human; Humans; Hybrid Cells; Leukemia, Myeloid - genetics; Male; man; Mice; myeloid leukemia; Promoter Regions, Genetic; Restriction Mapping; Sequence Homology, Nucleic Acid; Genetic mapping; Human; Cell proliferation; C9-Chromosome; Myeloid cell
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/S0888-7543(05)80388-X

The growth arrest-specific gene, Gas-1, was cloned from quiescent NIH3T3 mouse fibroblasts. Gas-1 mRNA accumulates when cells enter quiescence (Go) and expression is down-regulated by stimulation with serum or growth factors. DNA synthesis is inhibited when expression of Gas-1 is forced in normal or transformed NIH3T3 cells. Gas-1 encodes an integral membrane protein with two putative transmembrane domains flanking an extracellular region and with no significant similarities to any known proteins. The presence of an extracellular arginine-glycine-aspartic acid sequence suggests that the Gas-1 protein can associate with integrin-type receptors and may be involved in contact inhibition or in anchorage of the cells to the extracellular matrix. Since expression of Gas-1 is specific to quiescence, the Gas-1 protein may be required to sustain growth arrest or be involved in the control of differentiation. Thus, Gas-1 could act as a tumor suppressor gene by preventing uncontrolled proliferation. A human homologue to mouse Gas-1 has been isolated. The human gene, GAS1, is 85% homologous with the mouse gene at the 5' promoter regions. Since cell cycle-control elements are commonly conserved between species, it is likely that the human and mouse Gas-1 genes have the same function. We report here the localization of GAS1 to human chromosome arm 9q at bands q21.3-q22, confirming another localization by Schneider.