Effect of thrombin conjugation on hemostatic efficacy of PLGA mesh through reagent free surface modification

• Published in: Journal of industrial and engineering chemistry (Seoul, Korea) Vol. 118; pp. 101 - 108
• Main Authors: Lee, In Kyu, You, Su Jung, Yun, Young Jin, Kim, Jae Kwang, Yang, Dae Hyeok, Chun, Heung Jae, Ko, Jaehoon, Koh, Youngjoo
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 25.02.2023; 한국공업화학회
• Subjects: Hemostasis; PLGA; Reagent free surface modification; Schiff base reaction; Thrombin; 화학공학; Thrombin; PLGA; Reagent free surface modification; Hemostasis; Schiff base reaction
• ISSN: 1226-086X; 1876-794X
• DOI: 10.1016/j.jiec.2022.10.049

In this study, thrombin-conjugated PLGA (THR-PLGA) electrospun meshes were prepared through reagent free surface modification based on Schiff base reaction between amine groups in thrombin and carbonyls on the surface of PLGA meshes, and their feasibilities on the improvement of hemostasis were evaluated. In vitro studies were performed with chromogenic substrate and transglutaminase activity assays to evaluate the thrombin activity and FXIIIa activity of the THR-PLGA samples, respectively. A rat tail injury model was employed for measuring hemostatic time of the samples. The results showed that the hemostatic efficacy of the samples increased with increase in the content of THR on the surface of the samples. In addition, partial splenectomy was carried out in rat model to investigate the potential management of hemorrhage of THR-PLGA, as compared to Vicryl® and TachoSil®. The results showed that the inflammatory response was significantly reduced compared to Vicryl® and TachoSil®, and a dense fibrous layer was produced to protect the inside from laceration at 2 weeks of treatment. Moreover, neo pulps were appeared right below the fibrous capsule. These findings suggest that THR-PLGA samples prepared through the reagent free immobilization method deserves further advanced investigations for future clinical applications.