Halioglobus lutimaris sp. nov., isolated from coastal sediment

• Published in: International journal of systematic and evolutionary microbiology Vol. 68; no. 3; pp. 876 - 880
• Main Authors: Shi, Ming-Jing, Wang, Chong, Wang, Xu-Ting, Du, Zong-Jun
• Format: Journal Article
• Language: English
• Published: England 01.03.2018
• Subjects: coastal sediment; Halioglobus lutimaris; polyphasic taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.002601

A novel Gram-stain-negative, strictly aerobic, non-flagellated and rod-shaped bacterium, designated HF004 , was isolated from a marine sediment sample collected from the coast of Weihai, China. The strain grew optimally at 28 °C, pH 7.5-8.0 and in the presence of 2.0-3.0 % (w/v) NaCl. Based on the 16S rRNA gene sequence analysis, strain HF004 was a member of the genus Halioglobus, appearing to be closely related to Halioglobus pacificus (96.1 %) and Halioglobus japonicus (95.6 %). The major fatty acids were summed feature 3 (i.e. C16 : 1ω7c and/or iso-C15 : 0 2-OH), C17 : 1ω8c and C18 : 1ω7c. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The predominant respiratory quinone was Q-8. The DNA G+C content was 57.2 mol%. Cells of strain HF004 were rod-shaped and formed circular, mucous and beige-pigmented colonies on marine agar after incubation for 72 h at 28 °C. On the basis of phenotypic, genotypic and phylogenetic evidence, strain HF004 is presented as a novel species, for which the name Halioglobus lutimaris sp. nov. is proposed. The type strain is HF004 (=KCTC 42395 =MCCC 1H00127 ).