Caged nucleotides/nucleosides and their photochemical biology
Nucleotides and nucleosides are not only key units of DNA/RNA that store genetic information, but are also the regulators of many biological events of our lives. By caging the key functional groups or key residues of nucleotides with photosensitive moieties, it will be possible to trigger biological...
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| Published in | Organic & biomolecular chemistry Vol. 11; no. 45; pp. 7814 - 7824 |
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| Main Authors | , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
England
07.12.2013
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| Subjects | |
| Online Access | Get full text |
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| Summary: | Nucleotides and nucleosides are not only key units of DNA/RNA that store genetic information, but are also the regulators of many biological events of our lives. By caging the key functional groups or key residues of nucleotides with photosensitive moieties, it will be possible to trigger biological events of target nucleotides with spatiotemporal resolution and amplitude upon light activation or photomodulate polymerase reactions with the caged nucleotide analogues for next-generation sequencing (NGS) and bioorthogonal labeling. This review highlights three different caging strategies for nucleotides and demonstrates the photochemical biology of these caged nucleotides.
Photolabile nucleotides are important tools for studying many biological events and useful reagents for next-generation sequencing and labeling. |
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| Bibliography: | Lihe Zhang, Professor, School of Pharmaceutical Sciences, Peking University. Li-He Zhang gained his graduate diploma of medicinal chemistry in 1967 from Beijing Medical College. From 1981-1983, he worked as a research associate in the Department of Chemistry, University of Virginia, USA. From 1985, he became a professor at the School of Pharmaceutical Sciences, and was then appointed as dean of the School (1987-1999) and director of the Chemistry Division of NSFC (1999-2006). He is a member of CAS (1995) and is on the editorial boards of many scientific journals including OBC Jingjing Sun gained her BS from Shenyang Pharmaceutical University in 2012. She is now a PhD student in Prof. Xinjing Tang's group. Her research interest is the use of caged oligonucleotides for spatiotemporally photoregulating gene functions. Junzhou Wu gained his BS from Peking University in 2011. He is now a MS student in Prof. Xinjing Tang's group. His research interest is the use of caged nucleotides for the site-specific labeling of nucleic acids and cell imaging. His research interests include the chemistry of nucleosides and nucleotides, anticancer and antiviral drugs. and Xinjing Tang, Associate Professor, State Key Laboratory of Natural and Biomimetic Drugs and School of Pharmaceutical Sciences, Peking University. Xin-Jing Tang graduated from Shandong University (1997) and obtained his PhD degree from the Technical Institute of Physics and Chemistry, the Chinese Academy of Sciences (2002). In 2003, he moved to the Department of Chemistry at University of Pennsylvania as a postdoc and then research associate. In 2009, he became a faculty member of the School of Pharmaceutical Sciences, Peking University. His current research interests focus on nucleic acids and their photochemical biology, and light-induced drug release. Jinhao Zhang gained his BS from China Agricultural University in 2013, He is now a PhD student in Prof. Xinjing Tang's group. His research interest is the use of caged nucleosides for the site-specific incorporation of oligonucleotides and photomanipulate gene expression in cells. ChemMedChem. Yuan Wang gained his BS from Shenyang Pharmaceutical University in 2011. He is now a PhD student in Prof. Xinjing Tang's group. His research interest is the use of caged oligonucleotides to photomanipulate gene expression in zebrafish embryos and mammalian cells. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
| ISSN: | 1477-0520 1477-0539 |
| DOI: | 10.1039/c3ob41735b |