Labilibacter sediminis sp. nov., isolated from marine sediment

• Published in: International journal of systematic and evolutionary microbiology Vol. 70; no. 1; pp. 321 - 326
• Main Authors: Wang, Feng-Qing, Chen, Zhu-Jie, Yang, Jian-Min, Wang, Wei-Jun, Feng, Yan-Wei, Li, Zan, Sun, Guo-Hua
• Format: Journal Article
• Language: English
• Published: England 01.01.2020
• Subjects: Bacterial Typing Techniques; Bacteroidetes - classification; Bacteroidetes - isolation & purification; Base Composition; China; DNA, Bacterial - genetics; Fatty Acids - chemistry; Geologic Sediments - microbiology; Phospholipids - chemistry; Phylogeny; RNA, Ribosomal, 16S - genetics; Seawater - microbiology; Sequence Analysis, DNA; Vitamin K 2 - analogs & derivatives; Vitamin K 2 - chemistry; China; marine sediment; Labilibacter sediminis sp. nov; 16S rRNA gene; polyphasic taxonomy
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.003758

A Gram-stain-negative, rod-shaped and facultatively anaerobic strain, designated CG51 , was isolated from marine sediment collected from a coastal area in Weihai, PR China. Strain CG51 grew at 4-37 °C (optimum, 28-30 °C), with 1.0-6.0 % (w/v) NaCl (2.0-3.0 %) and at pH 6.0-8.5 (pH 7.0-7.5). The predominant fatty acids were iso-C , anteiso-C and iso-C . Major polar lipids included an unidentified lipid and a phospholipid. The respiratory quinone was MK-7 and the genomic DNA G+C content was 35.9 mol%. The results of phylogenetic analysis based on 16S rRNA gene sequences placed strain CG51 in the genus with the close relatives being Y11 and HQYD1 , exhibiting 96.5 and 96.3 % 16S rRNA pairwise similarity, values which are clearly below the 98.7 % threshold value recommended for species demarcation. Based on the phylogenetic, physiological, chemotaxonomic and genetic data, strain CG51 represents a novel species within the genus , for which the name sp. nov. is proposed. The type strain is CG51 (=MCCC 1K03739 =JCM 33138 ).