Extracellular diaphorase-like activity as a marker of cytolytic damage to cells

• Published in: Immunology letters Vol. 33; no. 3; pp. 223 - 228
• Main Authors: Petrov, Rem V., Kalinina, A.R., Sadovnikov, V.B., Vodyanik, M.A.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.08.1992; Elsevier
• Subjects: Biological and medical sciences; Biomarkers; Cell-mediated cytotoxicity; Cytotoxic reactions (adcc reaction, cell-mediated lympholysis, complement-dependent cytotoxicity and others); Cytotoxicity, Immunologic; Diaphorase; Dihydrolipoamide Dehydrogenase - metabolism; Extracellular Space - enzymology; Extracellular Space - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunobiology; Immunological reactions in vitro; Killer Cells, Lymphokine-Activated - immunology; Killer Cells, Natural - immunology; Kinetics; Lymphokine-activated killer; Natural killer; Tumor Cells, Cultured - immunology; Cell-mediated cytotoxicity; Diaphorase; Natural killer; Lymphokine-activated killer; Natural killer cell; Human; Effectory cell; Enzymatic activity; Investigation method; Enzyme; Dihydrolipoamide reductase (NAD+); Cytotoxicity; Target cell; Kinetics; Extracellular
• ISSN: 0165-2478; 1879-0542
• DOI: 10.1016/0165-2478(92)90065-V

A new variant of the enzyme release colorimetric method for NK/LAK cytotoxicity is described. Kinetic determination of diaphorase released from damaged target cells (TC) provided an exact measurement of the lytic activity of effector cells (EC). A polyenzyme testing system permitted the use of a wide spectrum of TC. The percentage cytotoxicity and the kinetics of the process coincide, within experimental error, with data from staining with Trypan blue. The method is simple and convenient and can be applied to study cell-mediated cytotoxicity (CMC).