Cellular influx of sulfobromophthalein by the biliary epithelium carcinoma cell line Sk-Cha-1 reveals kinetic criteria of a carrier-mediated uptake mechanism

• Published in: Biochimica et biophysica acta Vol. 1014; no. 2; pp. 108 - 111
• Main Authors: Stremmel, Wolfgang, Falbrede, Jörg, Diede, Hans Erich, Elsing, Christoph
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20.11.1989; Elsevier; North-Holland
• Subjects: Biliary epithelium carcinoma cell line; Biliary Tract Neoplasms - metabolism; Bilirubin - analogs & derivatives; Bilirubin - pharmacology; Bilirubin metabolism; Binding, Competitive; Biological and medical sciences; Biological Transport, Active - drug effects; Carrier mediated transport; Carrier Proteins - metabolism; Cell Membrane - metabolism; Cell physiology; Epithelium - metabolism; Fundamental and applied biological sciences. Psychology; Human liver; Humans; Kinetics; Membrane and intracellular transports; Molecular and cellular biology; Sulfobromophthalein - metabolism; Temperature; Tumor Cells, Cultured; Bilirubin metabolism; Biliary epithelium carcinoma cell line; Carrier mediated transport; BSP; Human liver; Ligand binding; Organic anion; Carcinoma; Cell line; Monolayer culture; Radiolabelling; Biological transport; Bilirubin; Kinetic parameter; Competitive inhibition; Metabolism; Bile
• ISSN: 0167-4889; 0006-3002; 1879-2596; 1878-2434
• DOI: 10.1016/0167-4889(89)90021-9

Cellular uptake of the cholephilic organic anion sulphobromophthalein (BSP) by the human biliary epithelium carcinoma cell line Sk-Cha-1 was examined at 37°C. In confluent monolayer cultures the cellular influx rate of increasing concentrations of [ 35S]BSP followed saturation kinetics with a K m value of 18 μM and a V max value of 243 pmol·min −1·mg protein −1. Uptake of [ 35S]BSP was competitively inhibited by the presence of bilirubin diglucuronide, but not by taurocholate or cholate. Furthermore, uptake was temperature dependent with maximal cellular influx rates at 37°C.