The repertoire of anti-TNP antibodies in mice neonatally suppressed with anti-IgM

• Published in: Cellular immunology Vol. 101; no. 2; pp. 604 - 612
• Main Authors: Gordon, Julius, Schotman, Els, Goidl, Edmond
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 01.09.1986; Elsevier
• Subjects: Animals; Animals, Newborn - immunology; Antibodies - classification; Antibodies - immunology; Antibodies, Anti-Idiotypic - immunology; Applied sciences; B-Lymphocytes - immunology; Exact sciences and technology; Ficoll - analogs & derivatives; Ficoll - immunology; Immunization; Immunoglobulin A - analysis; Immunoglobulin M - analysis; Immunoglobulin M - immunology; Immunosuppression; Mice; Mice, Inbred BALB C; Mice, Inbred C3H; Mice, Inbred C57BL; Other techniques and industries; Trinitrobenzenes - immunology
• ISSN: 0008-8749; 1090-2163
• DOI: 10.1016/0008-8749(86)90170-X

Previous studies have shown that mice immunized with TNP-Ficoll when young, adult, or aged expressed different repertoires of anti-TNP antibodies. The aim of the present study was to find out whether this age-related nonrandom progression was driven by antigen, and whether it was regulated by the immune network through surface-Ig receptors on B lymphocytes. The approach utilized was to block receptor expression on B lymphocytes of mice by the chronic administration of anti-IgM from birth for approximately 1 year, and then compare their subsequent antibody response to that of age-matched control animals. The results obtained have shown that the age-dependent shift in the anti-TNP repertoire expressed could take place in animals whose B lymphocytes were blind to antigen and anti-id for the greater part of their lives and thus suggest that the regulatory events responsible for this shift may be (surface Ig) receptor independent.