Lysobacter novalis sp. nov., isolated from fallow farmland soil

• Published in: International journal of systematic and evolutionary microbiology Vol. 65; no. 9; pp. 3131 - 3136
• Main Authors: Singh, Hina, Du, Juan, Won, Kyung-Hwa, Yang, Jung-Eun, Akter, Shahina, Kim, Ki-Young, Yi, Tae-Hoo
• Format: Journal Article
• Language: English
• Published: England 01.09.2015
• Subjects: Bacterial Typing Techniques; Base Composition; DNA, Bacterial - genetics; Fatty Acids - chemistry; Lysobacter - classification; Molecular Sequence Data; Nucleic Acid Hybridization - genetics; Phylogeny; Quinones - analysis; Republic of Korea; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; Soil; Soil Microbiology
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijsem.0.000389

A novel bacterial strain, designated THG-PC7(T), was isolated from fallow farmland soil in Yongin, South Korea. Cells of strain THG-PC7(T) were Gram-stain-negative, dark yellow, aerobic, rod-shaped and had gliding motility. Strain THG-PC7(T) grew optimally at 25-35 °C, at pH 7 and in the absence of NaCl. Comparative 16S rRNA gene sequence analysis identified strain THG-PC7(T) as belonging to the genus Lysobacter, exhibiting highest sequence similarity with Lysobacter ximonensis KCTC 22336(T) (98.7%) followed by Lysobacter niastensis KACC 11588(T) (95.7%). In DNA-DNA hybridization tests, DNA relatedness between strain THG-PC7(T) and its closest phylogenetic neighbour L. ximonensis was below 25%. The DNA G+C content of the novel isolate was determined to be 62.5 mol%. Flexirubin-type pigments were found to be present. The major cellular fatty acids were determined to be iso-C15 : 0, iso-C16 : 0, anteiso-C15 : 0 and iso-C17 : 1ω9c. The major respiratory quinone was identified as ubiquonone-8 (Q8). The predominant polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol and an unidentified aminophospolipid. On the basis of results from DNA-DNA hybridization and the polyphasic data, strain THG-PC7(T) represents a novel species of the genus Lysobacter, for which the name Lysobacter novalis sp. nov. is proposed. The type strain is THG-PC7(T)( = KACC 18276(T) = CCTCC AB 2014319(T)).