Synergism between pure Thermomonospora fusca and Trichoderma reesei cellulases

• Published in: Biomass & bioenergy Vol. 4; no. 4; pp. 293 - 299
• Main Authors: Bothwell, M.K., Walke, L.P., Wilson, D.B., Irwin, D.C., Price, M.
• Format: Journal Article
• Language: English
• Published: Elsevier Ltd 1993
• Subjects: cellobiohydrolase; Cellulase; endoglucanase; ratios; synergism; Thermomonospora fusca; Trichoderma reesei; endoglucanase; Cellulase; synergism; Thermomonospora fusca; cellobiohydrolase; ratios; Trichoderma reesei
• ISSN: 0961-9534; 1873-2909
• DOI: 10.1016/0961-9534(93)90088-L

Mixtures of Trichoderma reesei cellobiohydrolase I (CBHI) or crude enzyme extract and Thermomonospora fusca endoglucanases E 2 or E 3 were investigated to determine which gave maximum rates of hydrolysis, maximum reducing sugar concentration (RSC) at 4 h, and maximum degree of synergistic effect (DSE). Mixtures of T. fusca E 3 and T. reesei crude enzyme extract (mass ratios of 0.1 to 1) produced the highest RSC (0.020 mg ml −1) and fastest initial reaction rate (0.009 mg ml −1 h −1) of the mixtures investigated. With exception of the DSEs observed for mixtures of T. fusca E 2 and T. reesei CBHI, mixtures analyzed showed maximum RSC, rate of hydrolysis, and DSE over a range of enzyme mass ratios. These properties were sensitive to changes in the ratio outside of the optimal ratio range but were independent of the ratio within the optimal range. The DSE results of mixtures of T. fusca E 2 and T. reesei CBHI were not conclusive. The ratio ranges which produced the maximum RSC, initial reaction velocity, and DSE did not completely overlap for all mixtures investigated.